JGG 1984, 11(6): 423-433 DOI:   ISSN: 1673-8527 CN: 11-5450/R           

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Cloning and Expression of Streptomyces aureofaciens Promoters in Escherichia col i

Dong Kening

Institute of Microbiology,Academia Sinica,Beijing

Abstract

Sau3A restriction fragments from Strcptomyces aureofaciens chromosome DNA have b een cloned into BglII site of pGA46.Transforments were selected on medium that a llowed the selection of Tcr promoter-bearing plasmids.The size of DNA fragments from streptomyces was reduced by PstI digestion and rejoining.Subcloning of the streptomycete inserted fragment into pGA46 and pBR322 accomplished expression of the Tcr gene.Nuclease BAL-31 was used for further reduction of the size of stre ptomycete inserted fragment.Stretomycete derived insert was reduced to 100—200 bp in recombinant plasmid pB-8-1 and pB-8-9.Further decrease in the size destroy ed promoter function.

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Received 1900-01-01 Revised 1900-01-01 Online 1984-11-10 
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