|JGG 1984, 11(6): 411-415 DOI: ISSN: 1673-8527 CN: 11-5450/R|
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Construction of a Cloning Plasmid Vector pMM15
Lu Haoying, Du Lianying, Ma Xiankai
Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing
A plasmid vector pMM15 containing a lac UV5 promoter has been constructed.The pl asmid vector has a unique EcoRI restriction site downstream to the lac UV5 promo ter which controls the expression of a galactokinase gene in suitable host.Cloni ng of a DNA segment in the EcoRI site can be easily detected by the appearance o f white recombinant colonies in a background of red vector colonies on MacConkey galactose indicator plates.
|Received 1900-01-01 Revised 1900-01-01 Online 1984-11-10|
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