JGG 1984, 11(6): 411-415 DOI:   ISSN: 1673-8527 CN: 11-5450/R           

Current Issue | Archive | Search in JGG                                                            [Print]   [Close]
Article  

Construction of a Cloning Plasmid Vector pMM15

Lu Haoying, Du Lianying, Ma Xiankai

Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing

Abstract

A plasmid vector pMM15 containing a lac UV5 promoter has been constructed.The pl asmid vector has a unique EcoRI restriction site downstream to the lac UV5 promo ter which controls the expression of a galactokinase gene in suitable host.Cloni ng of a DNA segment in the EcoRI site can be easily detected by the appearance o f white recombinant colonies in a background of red vector colonies on MacConkey galactose indicator plates.

Keywords
Received 1900-01-01 Revised 1900-01-01 Online 1984-11-10 
DOI:
Fund:
Corresponding Authors:
Email:
About author:

References:
Similar articles

Copyright by JGG