JGG 1984, 11(6): 434-440 DOI:   ISSN: 1673-8527 CN: 11-5450/R           

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Molecular Cloning of the 2.17 kb EcoRI/BamHI Fragment of the mtDNA from Beijing Duck Liver

Zhao Bangti, Zhu Zhen, Ma Shuyi, Shen Tung

Department of Biology,Peking University

Abstract

The 2.17 kb EcoRI/BamHI restriction fragment of the mtDNA from Beijing duck live r was cloned,using plasmid pAT153 as a vector and E.coli SK1592 as recipient cel ls The recombinants have been identified by restriction analysis and Southern bl ot analysis.Moreover,the stability of the chimeric plasmids in recipient cells h as been studied and the question of nonsuccessful cloning of anotyer EcoRI/BamHI fragment (2.81 kb) of duck liver mtDNA is also discussed.

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Received 1900-01-01 Revised 1900-01-01 Online 1984-11-10 
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