JGG 2002, 29(11): 977-982 DOI:   ISSN: 1673-8527 CN: 11-5450/R           

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Research on Alu Element Inserted Mutation in Porcine FSH β Subunit Gene

DU Li-Xin1; LIU Shu-Fang2; YAN yan-Chun2; JIANG Yun-Liang1

1.College of Animal Science and Technology, Shandong Agricultural University, Taian 271018,China 2.College of Life Science, Shandong Agricultural University,Taian 271018,China

Abstract

The inserted fragment of FSH β subunit gene in Laiwu pigs, an excellent local pig breed in North China, Duli pigs and Landrace pigs was amplified, cloned and sequenced. Sequence analysis showed that the length of the fragments inserted between + 809bo and + 810bp of the published sequences (D00621) were 275bp,277bp and 274bp in Laiwu, Duli and Landrace pigs, respectively, And the poly (A) s in these inserted fragments were 17, 19 and 16 adenines, respectively. They were all shorter than those in Taihu pigs (292bp and 32 adenines, respectively) reported formerly. According to the RNA polymerase Ⅲ promoter structure and Alu I restriction enzyme site in the inserted fragment, it should be regarded as an Alu element. FSH β subunit gene was considered as one candidate gene of pig litter size triat, while DNA polymerase Ⅲ promoter could promote the transcription of the neighboring chromosome sequence so as to control the expression of FSH β subunit gene or other genes. As a result, the major difference of the inserted fragment among different pig breeds was originated from the length of poly (A) end. So it was assumed that the poly (A) structure in the serted fragment could influence the pig litter size. Because Laiwu pigs with genotype AA had 1.2 litter size more than those with genotype BB, it could be concluded that FSH β subunit gene was related with pig litter size traits or linked with the genes of which,and the poly (A) structure in this Alu element played a critical role.

Keywords pis   FSH β subunit gene   inserted mutation   Alu element   poly(A) structure  
Received 1900-01-01 Revised 1900-01-01 Online 2002-11-10 
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