JGG 2002, 29(11): 972-976 DOI:   ISSN: 1673-8527 CN: 11-5450/R           

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The Construction of the Genetic Map and QTL Locating Analysis on Chromosome 2 in Swine

QU Yan-Chun;DENG Chang-Yan;XIONG Yuan-Zhu;ZHENG Rong;YU Li;SU Yu-Hong;LIU Gui-Lan

Key Laboratory of Pig Breeding and Genetics, Agricultural Ministry, Huazhong Agricultural University, Wuhan 430070,China

Abstract

The study constructed the genetic linkage map of porcine chromosome 2 and further analysis of quantitative trait loci was conducted. The results of the study demonstrated that all 7 microsatellite loci we chose were with relatively high polymorphism, and its polymorphic information content was from 0.40182 to 0.58477. The genetic map we constructed for resource family was 152.9cM in length, with the order of all loci highly consistented with the USDA map. All marker intervals were longer than USDA map with the interval between marker Sw2516 and Sw1201 as an exception. Furthermore, we conducted QTLs locating analysis by combining the genetic map with the phenotypic data. QTLs affecting lively estimated traits such as lean meat percentage, were located at 60~65cM on chromosome 2, while QTLs for the height and marbling of Longissmus dorsi muscle were located at 20cM and 55cM, respectively Among them, QTL for estimated lean meat percentage was significant at chromosome-wise level (P < 0.01) and was responsible for 21.55% of the phenotypic variance. QTLs for the height and marbling of Longissmus dorsi muscle were responsible for 10.12% and 10.97% of the phenotypic variance, respectively. The additive and dominance effect of lively estimated traits were in the inverse tendency, while the QTL for the height of Longissmus dorsi muscle had its additive and dominance effect in the same tendency and was with advantageous allele in Large White. The QTLs we detected had relatively large effect on phenotype and built a basis for molecular marker assisted selection and breeding.

Keywords porcine   microsatellite   genetic map   quantitative trait loci  
Received 1900-01-01 Revised 1900-01-01 Online 2002-11-10 
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