Genetic Analysis and Linkage Mapping in a Resource Pig Population Using Microsatellite Markers

a.
Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
b.
Department of Biology Science & Technology, Zhangzhou Normal College, Zhangzhou 363000, China

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Corresponding author: E-mail address: xiongyzh@public.wh.hb.cn (Yuanzhu Xiong)

摘要

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Abstract: The use of markers and linkage map construction are important for QTL mapping in pigs. In this article, the genetic characteristics were studied and the linkage map was constructed in a pig resource population including 214 individuals by typing 39 microsatellite marker loci onSus scrofa chromosomes, SSC4, SSC6, SSC7, SSC8, and SSC13. Results indicated that the average allele number, the average observed heterozygosity (H_o), and the average polymorphism information content (PIC) in F₁ and F₂ population were 3.2, 0.528, 0.463 and 3.2, 0.496, 0.447, respectively. In the pig resource population, the average informative meiosis (IM) was 217.4 (44-316), and the average linkage map length between the two sexes on the five chromosomes were 172.3 cM (SSC4), 168.7 cM (SSC6), 191.7 cM (SSC7), 197.3 cM (SSC8), and 178.3 cM (SSC13). The orders of microsatellite marker loci in the linkage maps were identical to, but the length was greater than, those of USDA-MARC reference map. The results of this research showed the genetic relationship and genetic characteristics of the microsatellite markers in the pig resource family population, and the linkage map could be used to for QTL mapping in the subsequent study.
- pig /
- microsatellite marker /
- linkage map /
- heterozygosity

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参考文献(18)

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