Characterization of the Full-length cDNA, Chromosomal Localization, and Polymorphism of the Porcine RPLP0 Gene

Xiao Wu^{a, b},
Shulin Yang^a,
Zhengmao Zhu^a,
Shutang Feng^a,
Kui Li^{a
, ,}

a.
Department of Gene and Cell Engineering, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China
b.
Department of Animal Genetics and Breeding, School of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

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Corresponding author: E-mail address: kuili@iascaas.net.cn (Kui Li)

摘要

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Abstract: RPLP0 gene encodes the acidic ribosomal phosphoprotein large P0 subunit, which is a component of the 60S subunit. The full-length cDNA sequence of porcine RPLP0 was obtained from skeletal muscle of fetal pig cDNA library and deposited in GenBank. The nucleotide sequence and the predicted protein sequence shared high sequence identity with other mammalian homologues. A C/A single nucleotide substitution in exon 5 was detected as Csp6?polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) shows allele frequency diversity among Tongcheng, Xiaomeishan, Yushan, Large White, Landrace, and Duroc breeds. Analyses of somatic cell hybrid panel (SCHP) and radiation hybrid (IMpRH) panel showed that the RPLP0 gene was mapped to SSC 14q22-q24 and was closely linked to locus SW1321 (25 cR, LOD = 14.54).
- RPLP0 /
- porcine /
- IMpRH /
- SCHP /
- polymorphisms

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参考文献(14)

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