The Complete Mitochondrial Genome of Salt-water Crocodile (Crocodylus porosus) and Phylogeny of Crocodilians

a.
College of Life Sciences, Anhui Normal University, Wuhu 241000, China
b.
Molecular Systematics Laboratory, American Museum of Natural History, New York 10024, USA

More Information

Corresponding author: E-mail address: wuxb@mail.ahnu.edu.cn (Xiaobing Wu)

摘要

- /
Abstract: The nucleotide sequence of the complete mitochondrial DNA (mtDNA) molecule of the salt-water crocodile (Crocodylus porosus) was determined in this article. The molecule is 16,917 base pairs (bp) in length, and codes for 22 tRNAs, 13 protein-coding genes, 2 rRNAs, as well as a control region (D-loop), as is characteristic for mitochondrial genomes of other metazoans. The gene order conforms to that of other crocodilians sequenced, but the arrangement of some tRNA genes differs from other vertebrates. It shows that the gene order of crocodilians is remarkably conserved. In this study, the relationships among crocodilians were examined in the phylogenetic analysis based on the control conserved regions of 17 crocodilians. The results suggest that the gharial (Gavialis gangeticus) joins the false gharial (Tomistoma schlegelii) on a common branch, and then constitutes a sister group to traditional Crocodylidae. Thus, the result supports that G. gangeticus belongs to Crocodylidae. The analyses also suggest that the African slender-snouted crocodile (Crocodylus cataphractus) can be treated as an isolated genus, and constitutes a sister group to Crocodylus.
- mitochondrial genome /
- phylogeny

HTML全文

参考文献(29)

[1]	Densmore, LD
[2]	Densmore, LD, White, et al. The systematics and evolution of the Crocodilia as suggested by restriction endonuclease analysis of mitochondrial and nuclear ribosomal DNA Copeia, 3 (1991),pp. 602-615
[3]	Aggarwal, RK, Majumdar, et al. Genetic affinities among crocodilians as revealed by DNA fingerprinting with a Bkm-derived probe Proc Natl Acad Sci USA, 91 (1994),pp. 10606-10609
[4]	Gatesy, J, DeSalle, et al. Alignment-ambiguous nucleotide site and the exclusion of systematic data Mol Phylogenet Evol, 2 (1993),pp. 152-157
[5]	Axel, J, Anette, et al. J Mol Evol, 61 (2005),pp. 620-626
[6]	Brochu, CA Copeia, 3 (2000),pp. 657-673
[7]	White, PS, Densmore, et al.
[8]	Gatesy, J, Amato, et al. Combined support for wholesale taxic atavism in Gavialine crocodylians Syst Biol, 52 (2003),pp. 403-422
[9]	Anderson, S, Bankier, et al. Sequence and organization of the human mitochondrial genome Nature, 290 (1981),pp. 457-465
[10]	Wong, TW, Clayton, et al. Cell, 42 (1985),pp. 951-958
[11]	Saccone, C, Pesole, et al. The main regulatory region of mammalian mitochondrial DNA: structure-function model and evolutionary pattern J Mol Evol, 33 (1991),pp. 83-91
[12]	Arnason, U, Gullberg, et al. Cetacean mitochondrial DNA control region: sequences of all extant baleen whales and two sperm whale species Mol Biol Evol, 10 (1993),pp. 960-970
[13]	Douzery, E, Randi, et al. The mitochondrial control region of Cervidae: evolutionary patterns and phylogenetic content Mol Biol Evol, 14 (1997),pp. 1154-1166
[14]	Randi, E, Mucci, et al. Ammal Conservation, 4 (2001),pp. 1-11
[15]	Randi, E, Lucchini, et al. Mol Phylogenet Evol, 19 (2001),pp. 187-201
[16]	Shedlock, AM, Parker, et al. Evolution of the salmonid mitochondrial control region Mol Phylogenet Evol, 1 (1992),pp. 179-192
[17]	Sambrook, J, Russell, et al.
[18]	Janke, A, Arnason, et al. Mol Biol Evol, 14 (1997),pp. 1266-1272
[19]	Wu, XB, Wang, et al. Chinese Science Bulletin, 48 (2003),pp. 2050-2054
[20]	Janke, A, Erpenbeck, et al. Proc R Soc Lond B, 268 (2001),pp. 623-631
[21]	Thompson, JD, Gibson, et al. The clustalx windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools Nucleic Acids Res, 24 (1997),pp. 4876-4882
[22]	Rychlik, W, Rychlik, et al.
[23]	Kumar, S, Tamura, et al.
[24]	Ray, DA, Densmore, et al. The Crocodilian mitochondrial control region: general structure, conserved sequences and evolutionary implications J Exp Zool (Mol Dev Evol), 294 (2002),pp. 334-345
[25]	Swofford, DL
[26]	Zhong, J, Li, et al. Gene rearrangement of mitochondrial genome in the vertebrate Acta Genetica Sinica, 32 (2005),pp. 322-330
[27]	Ojala, D, Montoya, et al. tRNA punctuation model of RNA processing in human mitochondria Nature, 290 (1981),pp. 470-474
[28]	Frazer-Abel, AA, Hagerman, et al. Determination of the angle between the acceptor and anticodon stems of a truncated mitochondrial tRNA J Mol Biol, 285 (1999),pp. 581-593
[29]	Quinn, TW, Mindell, et al. Mitochondrial gene order adjacent to the control region in crocodile, turtle, and tuatara Mol Phylogenet Evol, 5 (1996),pp. 344-351