The Distribution of Repetitive DNAs Along Chromosomes in Plants Revealed by Self-genomic in situ Hybridization

Chaowen She^{a, b
, ,},
Jingyu Liu^{b, c},
Ying Diao^{b, d},
Zhongli Hu^b,
Yunchun Song^{b
, ,}

a.
Department of Biology, Huaihua University, Huaihua 418008, China
b.
Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, Wuhan 430072, China
c.
Human Genome Research Center and College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
d.
Department of Life sciences, Chongqing University of Arts and Sciences, Chongqing 400068, China

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Corresponding author: E-mail address: shechaowen@tom.com (Chaowen She); E-mail address: songyc1936@yahoo.com.cn (Yunchun Song)

摘要

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Abstract: The distribution of repetitive DNAs along chromosomes is one of the crucial elements for understanding the organization and the evolution of plant genomes. Using a modified genomic in situ hybridization (GISH) procedure, fluorescence in situ hybridization (FISH) with genomic DNA to their own chromosomes (called self-genomic in situ hybridization, self-GISH) was carried out in six selected plant species with different genome size and amount of repetitive DNA. Nonuniform distribution of the fluorescent labeled probe DNA was observed on the chromosomes of all the species that were tested. The signal patterns varied among species and were related to the genome size. The chromosomes of the small Arabidopsis genome were labeled almost only in the pericentromeric regions and the nucleolus organizer regions (NORs). The signals in the relatively small genomes, rice, sorghum, and Brassica oleracea var. capitata L., were dispersed along the chromosome lengths, with a predominant distribution in the pericentromeric or proximal regions and some heterochromatic arms. All chromosomes of the large genomes, maize and barley, were densely labeled with strongly labeled regions and weakly labeled or unlabeled regions being arranged alternatively throughout the lengths. In addition, enhanced signal bands were shown in all pericentromeres and the NORs in B. oleracea var. capitata and in all pericentromeric regions and certain intercalary sites in barley. The enhanced signal band pattern in barley was found consistent with the N-banding pattern of this species. The GISH with self-genomic DNA was compared with FISH with C_ot-1 DNA in rice, and their signal patterns are found to be basically consistent. Our results showed that the self-GISH signals actually reflected the hybridization of genomic repetitive DNAs to the chromosomes, thus the self-GISH technique would be useful for revealing the distribution of the regions where repetitive DNAs concentrate along chromosomes and some chromatin differentiation associated with repetitive DNAs in plants.
- plant genome /
- repetitive DNA /
- chromatin differentiation /
- genome organization

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[1]	Flavell, RB, Bennett, et al. Genome size and the proportion of repeated nucleotide sequence DNA in plants Biochem Genet, 12 (1974),pp. 257-269
[2]	Nature, 408 (2000),pp. 796-815
[3]	SanMiguel, P, Tikhonov, et al. Nested retrotransposons in the intergenic regions of the maize genome Science, 274 (1996),pp. 765-768
[4]	Shirasu, K, Schulman, et al. A contiguous 66 kb barley DNA sequence provides evidence for reversible genome expansion Genome Res, 10 (2000),pp. 908-915
[5]	Wicker, T, Stein, et al. Plant J, 26 (2001),pp. 307-316
[6]	SanMiguel, PJ, RamaKrishna, et al. Transposable elements, genes and recombination in a 215-kb contig from wheat chromosome 5A(m) Funct Integr Genomics, 2 (2002),pp. 70-80
[7]	Feng, Q, Zhang, et al. Sequence and analysis of rice chromosome 4 Nature, 420 (2002),pp. 316-320
[8]	Sasaki, T, Matsumoto, et al. The genome sequence and structure of rice chromosome 1 Nature, 420 (2002),pp. 312-316
[9]	Rice chromosome 10 sequencing consortium In-depth view of structure, activity, and evolution of rice chromosome 10 Science, 300 (2003),pp. 1566-1569
[10]	Feuillet, C, Keller, et al. High gene density is conserved at syntenic loci of small and large grass genomes Proc Natl Acad Sci USA, 96 (1999),pp. 8265-8270
[11]	Sandhu, D, Gill, et al. Gene-containing regions of wheat and the other grass genomes Plant Physiol, 128 (2002),pp. 803-811
[12]	Schmidt, T, Heslop-Harrison, et al. The physical and genomic organization of microsatellites in sugar beet Proc Natl Acad Sci USA, 93 (1996),pp. 8761-8765
[13]	Brandes, A, Heslop-Harrison, et al. Plant Mol Biol, 33 (1997),pp. 11-21
[14]	Vershinin, AV, Druka, et al. Plant Mol Biol, 49 (2002),pp. 1-14
[15]	Jiang, N, Bao, et al. Genetics, 161 (2002),pp. 1293-1305
[16]	Mroczek, RJ, Dawe, et al. Distribution of retroelements in centromeres and neocentromeres of maize Genetics, 165 (2003),pp. 809-819
[17]	Schmidt, T, Heslop-Harrison, et al. Genomes, genes and junk: the large-scale organization of plant chromosomes Trends Plant Sci, 3 (1998),pp. 195-199
[18]	Hennig, W Heterochromatin Chromosoma, 108 (1999),pp. 1-9
[19]	Avramova, ZV Heterochromatin in animals and plants: similarities and differences Plant Physiol, 129 (2002),pp. 40-49
[20]	Kapuscinski, J DAPI: A DNA-specific fluorescent probe Biotechnic Histochem, 70 (1995),pp. 220-233
[21]	Gill, BS
[22]	Le, HT, Armstrong, et al. Detection of rye DNA in wheat-rye hybrids and wheat translocation stocks using total genomic DNA as a probe Plant Mol Biol Rep, 7 (1989),pp. 150-158
[23]	Leitch, AR, Mosgöller, et al. J Cell Sci, 95 (1990),pp. 335-341
[24]	Schwarzacher, T, Leitch, et al. Ann Bot, 64 (1989),pp. 315-324
[25]	Arumuganathan, K, Earle, et al. Nuclear DNA content of some important plant species Plant Mol Biol Rep, 9 (1991),pp. 211-215
[26]	Song, YC, Liu, et al. Comparisons of G-banding patterns in six species of the Poaceae Hereditas, 121 (1994),pp. 31-38
[27]	Murray, MG, Thompson, et al. Rapid isolation of high molecular weight plant DNA Nucleic Acids Res, 8 (1980),pp. 4321-4325
[28]	Zwick, MS, Hanson, et al. Genome, 40 (1997),pp. 138-142
[29]	Schubert, I, Fransz, et al. Chromosome painting in plants Methods Cell Sci, 23 (2001),pp. 57-69
[30]	Fukui, K, Kakeda, et al. Quantitative karyotype of barley chromosomes by image analysis methods Genome, 33 (1990),pp. 450-458
[31]	Jensen, J, Linde-Laursen, et al. Statistical evaluation of length measurements on barley chromosomes with a proposal for new nomenclature for symbols and positions of cytogenetic markers Hereditas, 117 (1992),pp. 51-59
[32]	Marthe, F, Künzel, et al. Localization of translocation breakpoints in somatic metaphase chromosomes of barley Theor Appl Genet, 89 (1994),pp. 240-248
[33]	Pedersen, C, Rasmussen, et al. Genome, 39 (1996),pp. 93-104
[34]	Kuipers, AGJ, van Os, et al. Chromosome Res, 5 (1997),pp. 31-39
[35]	Bardsley, D, Cuadrado, et al. Theor Appl Genet, 99 (1999),pp. 300-304
[36]	Zoller, JF, Yang, et al. Chromosome Res, 9 (2001),pp. 357-375
[37]	Falistocco, E, Torricelli, et al. Theor Appl Genet, 105 (2002),pp. 829-833
[38]	Ali, HB, Lysak, et al. Genome, 47 (2004),pp. 954-960
[39]	Brandes, A, Thompson, et al. Chromosome Res, 5 (1997),pp. 238-246
[40]	Shibata, F, Hizume, et al. Chromosoma, 11 (2002),pp. 184-191
[41]	Heslop-Harrison, JS, Brandes, et al. Genetica, 100 (1997),pp. 197-204
[42]	Menz, MA, Klein, et al. Plant Mol Biol, 48 (2002),pp. 483-499
[43]	Islam-Faridi, MN, Childs, et al. Genetics, 161 (2002),pp. 345-353
[44]	Smith, DB, Flavell, et al. Characterisation of the wheat genome by renaturation kinetics Chromosoma, 50 (1975),pp. 223-242
[45]	Hake, S, Walbot, et al. Chromosoma, 79 (1980),pp. 251-270
[46]	Wang, XH, Luo, et al. Acta Bot Sin, 49 (1987),pp. 149-155
[47]	Harrison, GE, Heslop-Harrison, et al. Theor Appl Genet, 90 (1995),pp. 157-165
[48]	Dennis, ES, Gerlach, et al. Identical polypyrimidine-polypurine satellite DNAs in wheat and barley Heredity, 44 (1980),pp. 349-366
[49]	Ørgaard, M, Heslop-Harrison, et al. Ann Bot, 73 (1994),pp. 195-203
[50]	Song, YC, Liu, et al. Acta Bot Sin, 25 (1981),pp. 40-45

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doi: 10.1016/S1673-8527(07)60048-4

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The Distribution of Repetitive DNAs Along Chromosomes in Plants Revealed by Self-genomic in situ Hybridization

Corresponding author: E-mail address: shechaowen@tom.com (Chaowen She); E-mail address: songyc1936@yahoo.com.cn (Yunchun Song)

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doi: 10.1016/S1673-8527(07)60048-4

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The Distribution of Repetitive DNAs Along Chromosomes in Plants Revealed by Self-genomic in situ Hybridization

Corresponding author: E-mail address: shechaowen@tom.com (Chaowen She); E-mail address: songyc1936@yahoo.com.cn (Yunchun Song)

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