Molecular Mechanisms of Cyclic Nucleotide-Gated Ion Channel Gating

a.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
b.
Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

More Information

Corresponding author: E-mail address: fxshi@njau.edu.cn (Fangxiong Shi)

摘要

- /
- /
Abstract: Cyclic nucleotide-gated ion channels (CNGs) are distributed most widely in the neuronal cell. Great progress has been made in molecular mechanisms of CNG channel gating in the recent years. Results of many experiments have indicated that the stoichiometry and assembly of CNG channels affect their property and gating. Experiments of CNG mutants and analyses of cysteine accessibilities show that cyclic nucleotide-binding domains (CNBD) bind cyclic nucleotides and subsequently conformational changes occurred followed by the concerted or cooperative conformational change of all four subunits during CNG gating. In order to provide theoretical assistances for further investigation on CNG channels, especially regarding the disease pathogenesis of ion channels, this paper reviews the latest progress on mechanisms of CNG channels, functions of subunits, processes of subunit assembly, and conformational changes of subunit regions during gating.
- cyclic nucleotide-gated ion channel (CNG) /
- conformational change /
- gating

HTML全文

参考文献(45)

[1]	Distler, M, Biel, et al. Expression of cyclic nucleotide-gated cation channels in nonsensory tissues and cells Neuropharmacology, 33 (1994),pp. 1275-1282
[2]	Friedmann, NK Cyclic nucleotide-gated channels in nonsensory organs Cell Calcium, 27 (2000),pp. 127-138
[3]	Kaupp, UB The cyclic nucleotide-gated channels of vertebrate photoreceptors and olfactory epithelium Trends Neurosc, 14 (1991),pp. 150-157
[4]	Menin, A Cyclic nucleotide-gated channels in visual and olfactory transduction Biophys Chem., 55 (1995),pp. 185-196
[5]	Kaupp, UB, Seifert, et al. Cyclic nucleotide-gated ion channels Physiol Rev., 82 (2002),pp. 769-824
[6]	Wang, ZC, Huang, et al. Molecular structures, physiological roles and regulatory mechanism of cyclic nucleotide-gated ion channels Chin J Biochem Mol Biol., 22 (2006),pp. 282-288
[7]	Wang, ZC, Luo, et al. Involvement of cyclic nucleotide-gated ion channel in disease pathogenesis Prog Vet Medic., 27 (2006),pp. 1-4
[8]	Shammat, IM, Gordon, et al. Stoichiometry and arrangement of subunits in rod cyclic nucleotide-gated channels Neuron., 23 (1999),pp. 809-819
[9]	Weitz, D, Ficek, et al. Subunit Stoichiometry of the CNG Channel of Rod Photoreceptors Neuron., 36 (2002),pp. 881-889
[10]	Zheng, J, Trudeau, et al. Rod cyclic nucleotide-gated channels have a stoichiometry of three CNGA1 subunits and one CNGB1 subunit Neuron., 36 (2002),pp. 891-896
[11]	Zhong, H, Lai, et al. Selective heteromeric assembly of cyclic nucleotide-gated channels Proc Natl Acad Sci USA, 100 (2003),pp. 5509-5513
[12]	Zheng, J, Zagotta, et al. Stoichiometry and assembly of olfactory cyclic nucletide-gated channels Neuron., 42 (2004),pp. 411-421
[13]	Peng, CH, Rich, et al. Subunit configuration of heteromeric cone cyclic nucleotide-gated channels Neuron., 42 (2004),pp. 401-410
[14]	Liu, DT, Tibbs, et al. Subunit stoichiometry of cyclic nucleotide-gated channels and effects of subunit order on channel function Neuron., 16 (1996),pp. 983-990
[15]	Varnum, MD, Black, et al. Molecular mechanism for ligand discrimination of cyclic nucleotidegated channels Neuron., 15 (1995),pp. 619-625
[16]	Bradley, J, Reisert, et al. Regulation of cyclic nucleotide-gated channels Curr Opin Neurobiol., 15 (2005),pp. 343-349
[17]	Giorgetti, A, Nair, et al. Structural basis of gating of CNG channels FEBS Lett., 579 (2005),pp. 1968-1972
[18]	Trudeau, MC, Zagotta, et al. An Intersubunit interaction regulates yrafficking of rod cyclic nucleotide-gated channels and is disrupted in an inherited form of blindness Neuron., 34 (2002),pp. 197-207
[19]	Rosenbaum, T, Gordon, et al. Dissecting intersubunit contacts in cyclic nucleotide-gated ion channels Neuron., 33 (2002),pp. 703-713
[20]	Matulef, K, Flynn, et al. Molecular rearrangements in the ligand-binding domain of cyclic nucleotide-gated channels Neuron., 24 (1999),pp. 443-452
[21]	Matulef, K, Zagotta, et al. Multimerization of the ligand binding domains of cyclic nucleotide-cated channels Neuron., 36 (2002),pp. 93-103
[22]	Sun, ZP, Akabas, et al. Exposure of redidues in the cyclic nucleotide-gated channel pore P region structure and function in gating Neuron., 16 (1996),pp. 141-149
[23]	Becchetti, A, Gamel, et al. Cyclic nucleotide-gated channels Pore topology studied through the accessibility of reporter cysteines J Gen Physiol., 114 (1999),pp. 377-392
[24]	Liu, J, Siegelbaum, et al. Change of pore helix conformational state upon opening of cyclic nucleotide-gated channels Neuron., 28 (2000),pp. 899-909
[25]	Flynn, GE, Zagotta, et al. Conformational changes in S6 coupled to the opening of cyclic nucleotide-gated channels Neuron., 30 (2001),pp. 689-698
[26]	Simoes, M, Garneau, et al. Cysteine mutagenesis and computer modeling of the S6 region of an intermediate conductance IKCa channels J Gen Physiol., 120 (2002),pp. 99-116
[27]	Giorgetti, A, Carloni, et al. Molecular modelling of ion chanels: structural predictions Curr Opin Chem Biol., 7 (2003),pp. 150-156
[28]	Gordon, SE, Varnum, et al. Direct interaction between amino- and carboxyl-terminal domains of cyclic nucleotide-gated channels Neuron., 19 (1997),pp. 431-441
[29]	Peng, C, Rich, et al. Functionally important calmodulin binding sites in both N- and Cterminal regions of the cone photoreceptor cyclic nucleotide-gated channel CNGB3 subunit J Biol Chem., 278 (2003),pp. 24617-24623
[30]	Peng, C, Rich, et al. Achromatopsiaassociated mutation in the human cone photoreceptor cyclic nucleotide-gated channel CNGB3 subunit alters the ligand sensitivity and pore properties of heteromeric channels J Biol Chem., 278 (2003),pp. 34533-34540
[31]	Kwon, RJ, Ha, et al. Binding symmetry of extracellular divalent cations to conduction pore studied using tandem dimers of a CNG channel Biochem Biophys Res Commun., 29 (2002),pp. 478-485
[32]	Zheng, J, Zagotta, et al. Gating rearrangements in cyclic nucleotide-gated channels revealed by patch-clamp fluorometry Neuron., 28 (2000),pp. 369-374
[33]	Johnson, J, Zagotta, et al. Rotation movement during cyclic nucleotide-gated channel opening Nature., 412 (2001),pp. 917-921
[34]	Ruiz, M, Karpen, et al. Opening mechanism of a cyclic nucleotide-gated channel based on analysis of single channels locked in each liganded state J Gen Physiol., 113 (1999),pp. 873-895
[35]	He, XL, Wang, et al. Detection Pb ofCd, Cu and Cr in chicken tissues by graphite furnace atomic absorption spectrometry Animal Husbandry and Veterinary Medicine, 37 (2005),pp. 15-17
[36]	Tao, SH, Han, et al. Effect of ipriflavone on growth performance and serum biochemical parameters in broilers Animal Husbandry and Veterinary Medicine, 39 (2007),pp. 18-21
[37]	Wang, ZC, Shi, et al. Phosphodiesterase 4 and compartmentalization of cyclic AMP signaling Chin Sci Bull., 52 (2007),pp. 34-46
[38]	Kruse, LS, Sandholdt, et al. Phosphodiesterase 3 and 5 and cyclic nucleotide-gated ion channel expression in rat trigeminovascular system Neurosci Lett., 404 (2006),pp. 202-207
[39]	Brown, RL, Strassmaier, et al. The pharmacology of cyclic nucleotide-gated channels: emerging from the darkness Curr Pharm Des., 12 (2006),pp. 3597-3613
[40]	Michalakis, S, Reisert, et al. Loss of CNGB1 protein leads to olfactory dysfunction and subciliary cyclic nucleotide-gated channel trapping J Biol Chem., 281 (2006),pp. 35156-35166
[41]	Qu, W, Moorhouse, et al. A single P-loop glutamate point mutation to either lysine or arginine switches the cation-anion selectivity of the CNGA2 channel J Gen Physiol., 127 (2006),pp. 375-389
[42]	Liu, C, Varnum, et al. Functional consequences of progressive cone dystrophy-associated mutations in the human cone photoreceptor cyclic nucleotide-gated channel CNGA3 subunit Am J Physiol Cell Physiol., 289 (2005),pp. C187-C198
[43]	Michalakis, S, Geiger, et al. Impaired opsin targeting and cone photoreceptor migration in the retina of mice lacking the cyclic nucleotide-gated channel CNGA3 Invest Ophthalmol Vis Sci., 46 (2005),pp. 1516-1524
[44]	Nishiguchi, KM, Sandberg, et al. Cone cGMP-gated channel mutations and clinical findings in patients with achromatopsia, macular degeneration, and other hereditary cone diseases Hum Mutat., 25 (2005),pp. 248-258
[45]	KA, Patel, KM, et al. Transmembrane S1 mutations in CNGA3 from achromatopsia 2 patients cause loss of function and impaired cellular trafficking of the cone CNG channel Invest Ophthalmol Vis Sci, 46 (2005),pp. 2282-2290