‘Genetic Diversity of Microsatellite DNA Loci of Tibetan Antelope (Chiru, Pantholops hodgsonii) in Hoh Xil National Nature Reserve, Qinghai, China

Hui Zhou^{a, b},
Diqiang Li^{a
, ,},
Yuguang Zhang^a,
Tao Yang^b,
Yi Liu^b

a.
Institute of Forestry Ecology, Environment and Protection, and the Key Laboratory of Forest Ecology and Environment of State Forestry Administration, Chinese Academy of Forestry, Beijing 100091, China
b.
Hunan Agricultural University, Changsha 410128, China

More Information

Corresponding author: E-mail address: lidq@caf.ac.cn (Diqiang Li)

摘要

- /
- /
Abstract: The Tibetan antelope (Pantholops hodgsonii), indigenous to China, became an endangered species because of considerable reduction both in number and distribution during the 20th century. Presently, it is listed as an Appendix?species by CITES and as Category? by the Key Protected Wildlife List of China. Understanding the genetic diversity and population structure of the Tibetan antelope is significant for the development of effective conservation plans that will ensure the recovery and future persistence of this species. Twenty-five microsatellites were selected to obtain loci with sufficient levels of polymorphism that can provide information for the analysis of population structure. Among the 25 loci that were examined, nine of them showed high levels of genetic diversity. The nine variable loci (MCM38, MNS64, IOBT395, MCMAI, TGLA68, BM1329, BMS1341, BM3501, and MB066) were used to examine the genetic diversity of the Tibetan antelope (n = 75) in Hoh Xil National Nature Reserve (HXNNR), Qinghai, China. The results obtained by estimating the number of population suggested that all the 75 Tibetan antelope samples were from the same population. The mean number of alleles per locus was 9.4 ? 0.5300 (range, 7-12) and the mean effective number of alleles was 6.519 ? 0.5271 (range, 4.676-9.169). The observed mean and expected heterozygosity were 0.844 ? 0.0133 (range, 0.791-0.897) and 0.838 ? 0.0132 (range, 0.786-0.891), respectively. Mean Polymorphism Information Content (PIC) was 0.818 ? 0.0158 (range, 0.753-0.881). The value of Fixation index (Fis) ranged from ?0.269 to ?0.097 with the mean of ?0.163 ? 0.0197. Mean Shannon's information index was 1.990 ? 0.0719 among nine loci (range, 1.660-2.315). These results provide baseline data for the evaluation of the level of genetic variation in Tibetan antelope, which will be important for the development of conservation strategies in future.
- genetic diversity /
- microsatellite locus /
- Tibetan antelope

HTML全文

参考文献(20)

[1]	Schaller, GB
[2]	Xu, SQ, Yang, et al. Genomics Proteomics Bioinformatics, 3 (2005),pp. 5-17
[3]	Rawling, C
[4]	Gatesy, J, Amato, et al. A clasdistic analysis of mitochondrial DNA from the Bovidae Mol Phylogenet Evol, 7 (1997),pp. 303-319
[5]	Zhigang, J, Diqiang, et al. Antelopes of China Izvestiia Akademiinauk: Seriia biologicheskaia, 4 (1998),pp. 458-461
[6]	Zhen, ZC Study on the fleece characteristics of Tibetan antelope China Herbivores, 04 (2001),pp. 14-16
[7]	Tian, FY, Liu, et al. The crania anatomic structure of Tibetan antelope Chin J Vet Sci Technol, 31 (2001),pp. 42-43
[8]	Tautz, D Hypervariability of simple sequences as a general source for polymorphic DNA markers Nucleic Acids Res, 17 (1989),pp. 6463-6471
[9]	Weber, JL, May, et al. Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction Am J Hum Genet, 44 (1989),pp. 388-396
[10]	Gouin, N, Westenberger, et al. Genome, 48 (2005),pp. 1019-1027
[11]	Zhou, H, Zhang, et al. Study on mitochondrial DNA genetic diversity of Tibetan Antelope Hereditas (Beijing), 28 (2006),pp. 299-305
[12]	Shi, R, Guo, et al. Introduction of silver staining of DNA in polyacrylamide gds Biotechnology, 8 (1998),pp. 46-48
[13]	Bostein, D, White, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms Am J Hum Genet, 32 (1980),pp. 314-331
[14]	Zhang, Yu-Guang, Li, et al. Genetic diversity of microsatellite loci in captive Amur tigers Hereditas, 26 (2004),pp. 620-624
[15]	Smith, DA, Ralls, et al. Assessing reliability of microsatellite genotypes from kit fox faecal samples using genetic and GIS analyses Mol Ecol, 15 (2006),pp. 387-406
[16]	Zhang, ZH, Shen, et al. Paternity assignment of giant panda by microsatellite genotyping Hereditas, 25 (2003),pp. 504-510
[17]	Walker, FM, Sunnucks, et al. Genotyping of “captured” hairs reveals burrow-use and ranging behavior of southern hairy-nosed wombats J Mammal, 87 (2006),pp. 690-699
[18]	Taberlet, P, Griffin, et al. Reliable genotyping of samples with very low DNA quantities using PCR Nucleic Acids Res, 24 (1996),pp. 3189-3194
[19]	Taberlet, P, Waits, et al. Noninvasive genetic sampling: look before you leap Trends Ecol Evol, 14 (1999),pp. 323-327
[20]	Smith, KL, Alberts, et al. Am J Primatol, 51 (2000),pp. 219-227