Genetic Analysis and Mapping of Genes Involved in Fertility of Pingxiang Dominant Genic Male Sterile Rice

Tingyou Huang^{a, b, c},
Yuping Wang^{a, b},
Bingtian Ma^{a, b},
Yuqing Ma^{a, b},
Shigui Li^{a, b
, ,}

a.
Rice Research Institute of Sichuan Agricultural University, Chengdu 611130, China
b.
Key Laboratory of Crop Genetic Resource and Improvement of Ministry of Education, Sichuan Agricultural University, Ya'an 625014, China
c.
Mianyang Agriculture Science Research Institute, Mianyang 621023, China

More Information

Corresponding author: E-mail address: Lishigui_sc@263.net (Shigui Li)

摘要

- /
- /
- /
Abstract: Pingxiang dominant genic male sterile rice (PDGMSR) was the first dominant genic male sterile mutant identified in rice (Oryza sativa L.), and the corresponding dominant genic male sterile gene was designated as Ms-p. The fertility of PDGMSR can be restored by introduction of a dominant epistatic fertility restoring gene in some rice varieties. In the present study, E823, an indica inbred rice variety, restored the fertility of PDGMSR, and the genetic pattern was found to be consistent with a dominant epistatic model, therefore, the dominant epistatic fertility restorer gene was designated asRfe. The F2 population from the cross of PDGMSR/E823 was developed to map gene Rfe. The F2 plants with the genotypes Ms-pMs-pRferfe or Ms-pms-pRferfe were used to construct a fertile pool, and the corresponding sterile plants with genotypes Ms-pMs-prferfe or Ms-pms-prferfe were used to construct a sterile pool. The fertility restoring gene Rfe was mapped to one side of the microsatellite markers RM311 and RM3152 on rice chromosome 10, with genetic distances of 7.9 cM and 3.6 cM, respectively. The microsatellite markers around the location of the Ms-p gene were used to finely map the Ms-p gene. The findings of this study indicated that the microsatellite markers RM171 and RM6745 flanked the Ms-p gene, and the distances were 0.3 cM and 3.0 cM, respectively. On the basis of the sequence of rice chromosome 10, the physical distance between the two markers is approximately 730 kb. These findings facilitates molecular marker-assisted selection (MAS) of genes Ms-p and Rfe in rice breeding programs, and cloning them in the future.
- dominant epistatic fertility restoring gene /
- dominant genic male sterile /
- gene mapping /
- PDGMSR

HTML全文

参考文献(22)

[1]	Liu, BH A discussion on classification and origin of dominant gene for male sterility in cultivated plant Hereditas, 15 (1993),pp. 32-34
[2]	Li, SG, Zhou, et al. Molecular mapping and genetic analysis of dominant themo-sensitive genic male sterile in rice Chin Sci Bull, 44 (1999),pp. 955-958
[3]	Yan, LA, Zhang, et al. The preliminary evaluation of dominant male sterile gene in rice Acta Agronom Sin, 15 (1989),pp. 174-180
[4]	He, HH, Liu, et al. Inheritance of dominant genic sterile and its restoration in rice Chin J Rice Sci, 13 (1999),pp. 143-146
[5]	Chen, XW, Li, et al. Mapping of the dominant genic sterile gene in pingxiang dominant genic sterile gice (PDGMSR) Chin Sci Bull, 45 (2000),pp. 1644-1647
[6]	Chen, DH, Ronald, et al. A rapid DNA minipreparation method suitable for AFLP and other PCR applications Plant Mol Biol Rep, 17 (1999),pp. 53-57
[7]	Lander, ES, Green, et al. MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations Genomics, 1 (1987),pp. 174-181
[8]	Gong, HM, He, et al. Critical temperature and the thermo-sensitive phase of pingxiang dominant genic male sterile rice Chin J Rice Sci, 14 (2000),pp. 19-23
[9]	Liu, DF Discussion on the inheritance of fertility restoring for the dominant genic male sterility in plant Hereditas, 14 (1992),pp. 31-36
[10]	McCouch, SR, Teytelman, et al. DNA Res, 9 (2002),pp. 199-207
[11]	Yao, FY, Xu, et al. Euphytica, 98 (1997),pp. 183-187
[12]	Liu, XQ, Xu, et al. Mol Genet Genomics, 271 (2004),pp. 586-594
[13]	Akagi, H, Nakamura, et al. Theor Appl Genet, 108 (2004),pp. 1449-1457
[14]	Dong, NV, Subudhi, et al. Molecular mapping of a rice gene conditioning thermosensitive genic male sterility using AFLP, RFLP and SSR techniques Theor Appl Genet, 100 (2000),pp. 727-734
[15]	Wang, YG, Xing, et al. Theor Appl Genet, 107 (2003),pp. 917-921
[16]	Lee, DS, Chen, et al. Theor Appl Genet, 111 (2005),pp. 1271-1277
[17]	Lang, NT, Subudhi, et al. Rice Genet Newslett, 14 (1997),pp. 102-104
[18]	Yamaguchi, Y, Ikeda, et al. Breed Sci, 47 (1997),pp. 371-373
[19]	Mei, MH, Dai, et al. Mapping and genetic analysis of the genes for photoperiod-sensitive genic male sterility in rice using the original mutant Nongken 58S Crop Sci, 39 (1999),pp. 1711-1715
[20]	Wang, B, Xu, et al. Tagging and mapping the thermo-sensitive genic male-sterile gene in rice with molecular markers Theor Appl Genet, 91 (1995),pp. 1111-1114
[21]	Reddy, OUK, Siddiq, et al. Genetic analysis of temperature sensitive male sterility in rice Theor Appl Genet, 100 (2000),pp. 794-801
[22]	Jia, JH, Zhang, et al. Theor Appl Genet, 103 (2001),pp. 607-612