Analysis on the Origin and Phylogenetic Status of Tong Sheep Using 12 Blood Protein and Nonprotein Markers

a.
Animal Science & Technology College, Yangzhou University, Yangzhou 25009, China
b.
Showa University School of Medicine, Tokyo 142-8555, Japan
c.
Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China
d.
Faculty of Veterinary Science, University of Nyala, Nyala 155, Sudan

More Information

Corresponding author: E-mail address: hoch@yzcn.net (Hong Chang)

摘要

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Abstract: This study is based on the Tong sheep obtained by the random sampling method of typical colonies in the central area of Baishui County in Shaanxi Province, China. An investigation was undertaken to clarify the gene constitution of blood protein and nonprotein types of Tong sheep. Twelve genetic markers were examined by starch-gel electrophoresis and cellulose acetate electrophoresis. Polymorphism in Tong sheep was found at the following 10 loci, transferrin (Tf), alkaline phosphatase (Alp), leucine aminopeptidase (Lap), arylesterase (Ary-Es), hemoglobin-β (Hb-β), X-protein (X-p), carbonic anhydrase (CA), catalase (Cat), malate dehydrogenase (MDH), and lysine (Ly), whereas, albumin (Al) and postalbumin (Po) loci were monomorphic. Genetic approach degree method and phylogenetic relationship clustering method were used to judge the origin and phylogenetic status of Tong sheep. Results from both methods maintained that Tong sheep belonged to the “Mongolia group”, and Mongolia sheep was the origin of Tong sheep. This was also supported by the history of Tong sheep breeding. Compared to the phylogenetic relationship clustering method, the genetic approach degree method was more reliable for the extraction from East and South of Central Asia, and was more effective in reflecting the breeding course of Tong sheep.
- Tong sheep /
- origin /
- phylogenetic status /
- genetic approach degree /
- phylogenetic relationship clustering

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参考文献(21)

[1]	Chang, H
[2]
[3]	Sun, W
[4]	Tsunoda, K, Amano, et al. Morphological characters and blood protein polymorphism of sheep in Bangladesh and genetic relationship with European sheep breeds Rep Soc Res Native Livestock, 12 (1988),pp. 161-185
[5]	Tsunoda, K, Doge, et al. Morphological traits and blood protein variation of the native Nepalese sheep Rep Soc Native Livestock, 14 (1992),pp. 155-183
[6]	Tsunoda, K, Nozawa, et al. Blood protein variation of native sheep population in Lufeng and Lunan in Yunnan province of China Rep Soc Res Native Livestock, 15 (1995),pp. 119-129
[7]	Tsunoda, K, Okabayashi, et al. Morphologic and genetic charactieristics of sheep raised by the cham tribe in Vietnam Rep Soc Res Native Livestock, 16 (1998),pp. 63-73
[8]	Tsunoda, K, Nozawa, et al. External morphological characters and blood protein and non-protein polymorphisom of native sheep in central Mongolia Rep Soc Res Native Livestock, 17 (1999),pp. 63-82
[9]	Tsunoda, K, Amano, et al. Morphological traits and biochemical polymorphisms of Myanmar sheep Rep Soc Res Native Livestock, 21 (2004),pp. 155-169
[10]	Tsurnoda, K, Amano, et al. Genetic characteristics of Bangladeshi sheep as based on biochemical variations Jpn J Zootech Sci, 61 (1990),pp. 54-66
[11]	Chang, H
[12]	Sun, W, Chang, et al. Study on genetic relationship of sheep populations from east and south of central Asia Asian-Aus J Anim Sci, 15 (2002),pp. 1398-1402
[13]	Lou, SB
[14]	Rohlf J F. NTSYSpc: numerical taxonomy and mulitivariate analysis system. Version 2.1, Users Guide. Exeter Software, Setauket, New York, 2000.
[15]	Lei, ZQ Tong sheep in Shaanxi Journal of Animal Science and Veterinary Medicine, 18 (1999),pp. 35-36
[16]	Liao, XJ, Chang, et al. Study of genetic diversity in six sheep/goat populations with microsatellite markers Acta Ecologiae Animalis Domastici, 27 (2006),pp. 13-18
[17]	Lei, XQ, Xu, et al. Microsatellite markers on genetic relationships of six Chinese indigenous sheep breeds Chinese Journal of Biochemistry and Molecular Biology, 22 (2006),pp. 81-85
[18]	Li, XL, Zhang, et al. Study on mtDNA D-loop of Chinese main indigenous sheep breeds using PCR-RFLP Hereditas (Beijing), 28 (2006),pp. 165-170
[19]	Gong, YF, Li, et al. Acta Veterinaria et Zootechnica Sinica, 36 (2005),pp. 649-653
[20]	Li, XL, Gong, et al. Study on polymorphisms of microsatellites DNA of six Chinese indigenous sheep breeds Acta Genet Sin, 31 (2004),pp. 1203-1210
[21]	Li, ZN