Effects of Downregulation of Inhibin α Gene Expression on Apoptosis and Proliferation of Goose Granulosa Cells

a.
College of Animal Science, Huazhong Agricultural University, Wuhan 430070, China
b.
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

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Corresponding author: E-mail address: xunping@gmail.com (Xunping Jiang)

摘要

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Abstract: Inhibin α is one of the candidate genes that control the ovulation in poultry. To study the genetic effects of inhibin α on apoptosis and proliferation of goose granulosa cells cultured in vitro, two RNA interference (RNAi) expression vectors, psiRNA-INHα1 and psiRNA-INHα2, were constructed to knock down inhibin α gene expression. After 48 h of transfection, the efficiency of these two RNAi expression vectors was examined by fluorescence microscopy. Meanwhile, inhibin protein expression levels, apoptosis indexes (AI) and proliferation indexes (PI) of granulosa cells were analyzed by flow cytometry. In addition, the supernatants were collected to assay the concentrations of estrogen (E₂) and progesterone (P) by radioimmunoassay. The results showed that the expression level of inhibin α in the RNAi group were decreased 30%–40% than those in the control groups (P < 0.05) and the apoptosis indexes and proliferation indexes in the RNAi groups were significantly higher than those in the control groups ( P < 0.05). However, the E ₂ concentrations in the RNAi groups were lower than those in the control groups (P < 0.05). These results indicate that inhibin α has antagonistic effect on granulosa cell apoptosis.
- inhibin α /
- RNAi /
- granulosa cell apoptosis /
- proliferation /
- goose

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参考文献(30)

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