Microsatellite analysis of genetic diversity and population structure of Chinese mitten crab (Eriocheir sinensis)

Yumei Chang^{a, b},
Liqun Liang^a,
Haitao Ma^{a, c},
Jianguo He^b,
Xiaowen Sun^{a
, ,}

a.
Key Laboratory of Finfish Bioengineering & Breeding in North China, Ministry of Agriculture, Heilongjiang River Fisheries, Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China
b.
College of Life Sciences, Zhongshan University, Guangzhou 510275, China
c.
Life Science of Technology Institute, Dalian Fisheries University, Dalian 116023, China

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Corresponding author: E-mail address: sunxw2002@163.com (Xiaowen Sun)

摘要

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Abstract: Chinese mitten crab (Eriocheir sinensis) has higher commercial value as food source than any other species of Eriocheir in China. To evaluate the germplasm resources and characterize the genetic diversity and population structure of the crabs in different water systems, two stocks and two farming populations were assessed with 25 polymorphic microsallite loci available in public GenBank. Basic statistics showed that the average observed heterozygosity (Ho) amongst populations ranged from 0.5789 to 0.6824. However, a remarkable presence of inbreeding and heterozygote deficiencies were observed. To analyze population structure, pairwise coefficients explained only ∼ 10.3% variability from the subdivision of mitten crab populations, the remaining variability stems from the subdivision within subpopulations. Although the four populations had slight differentiation, different allelic frequencies resulted in distinct population structures. Two stocks and one farming population were clustered together to the phylogenetic branch of Yangtze crab, with an approximate membership of 95%. Whereas, another farming population was clustered singly to the phylogenetic branch of the Liaohe crab, with a membership of 97.1%. The tests for individual admixture showed that Yangtze crab had probably been contaminated with individuals from other water systems. Genetic relationships between populations also supported the conclusion that Yangtze crab and Liaohe crab had different gene pools in spite of the origins of the same species.
- Chinese mitten crab /
- genetic diversity /
- population structure /
- admixture

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