Effects of donor cells on in vitro development of cloned bovine embryos

Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200040, China

More Information

Corresponding author: E-mail address: f.zeng@verizon.net (Fanyi Zeng); E-mail address: ytzeng@stn.sh.cn (Yitao Zeng)

摘要

- /
- /
Abstract: The donor cells from different individuals and with different foreign genes introduced were investigated to determine their effects on the efficiency of somatic cell nuclear transfer (SCNT). The bovine ear fibroblast from different individuals was isolated, cultured, and then transfected with foreign genes to establish the stable cell lines, which were used as donor cells for nuclear transfer. The oocytes were obtained through ovum pick up operation. Afterin vitro maturation, the M II phase oocytes were selected as receptors for nuclear transfer. The reconstructed embryos were cultured in vitro and observed at 2 h, 48 h, and 7 days after transfer to assess the rate of fusion using cleaved and blastocyst as the parameters of SCNT efficiency. The donor cells from different individuals (04036, 06081, 06088, and 06129) had no obvious effect on the fusion and cleaved rate, whereas there was significant difference in the blastocyst rate (P<0.05), and the rate was 62.3%, 37.0%, 35.1%, and 15.6%, respectively. There was no significant difference among the rate of fusion, cleaved and blastocyst in donor cells with different foreign genes (P>0.05). It was concluded that the genetic background of the donor cells could affect the efficiency of SCNT, while the introduction of foreign genes into the donor cells had no obvious effect on the efficiency. This study provides useful information for the SCNT and would benefit in promoting the efficiency.
- donor cell /
- bovine somatic nuclear transfer /
- transgene

HTML全文

参考文献(22)

[1]	Arat, S., Rzucidlo, S.J., Gibbons, J. et al. Production of transfected bovine embryos by transfer of transfected granulosa cells into enucleated oocytes Mol. Reprod. Dev., 60 (2001),pp. 20-26
[2]	Barritt, J.A., Kokot, M., Cohen, J. et al. Quantification of human ooplasmic mitochondria Reprod. Biomed., 4 (2002),pp. 243-247
[3]	Bhuiyan, M.M.U., Cho, J., Jang, G. et al. J. Vet. Med. Sci., 66 (2004),pp. 257-261
[4]	Dominko, T., Chan, A., Simerly, C. et al. Dynamic imaging of the metaphase II spindle and maternal chromosomesin bovine oocytes: Implications for enucleation efficiency verification, avoidance of parthenogenesis, and successful embryogenesis Biol. Reprod., 62 (2000),pp. 150-154
[5]	Han, Y.M., Koo, D.B., Kang, Y.K. et al. Production of a transfected calf by nuclear transfer using transformed cells Theriogenology, 55 (2001),p. 270
[6]	Hill, J.R., Roussel, A.J., Cibelli, J.B. et al. Clinical and pathologic features of cloned transfected calves and fetuses (13 case studies) Theriogenology, 51 (1999),pp. 1451-1465
[7]	Hodges, C.A., Stice, S.L. Generation of bovine transfected using somatic cell nuclear transfer Reprod. Biol. Endocrinol., 1 (2003),p. 81
[8]	Hua, S., Zhang, Z.P., Zhang, C. et al. An improved enucleation method of bovine somatic cell nuclear transfer J. Genet. Genomics, 34 (2007),pp. 491-496
[9]	Huang, S.Z., Huang, Y., Chen, M.J. et al. Theriogenology, 56 (2001),pp. 545-556
[10]	Huang, S.Z., Yan, J.B., Huang, Y. et al. Acta Genet. Sin., 29 (2002),pp. 206-211
[11]	Knott, J.G., Poothapillai, K., Wu, H. et al. Porcine sperm factor supports activation and development of bovine nuclear transfer embryos Biol. Reprod., 66 (2002),pp. 1095-1103
[12]	Jiao, F., Yan, J.B., Yang, X.Y. et al. Effect of oocytes mitochondrial DNA haplotype on bovine smatic cell nuclear transfer efficiency Mol. Reprod. Dev., 74 (2007),pp. 1278-1286
[13]	Kato, Y., Tani, T., Sotomaru, Y. et al. Eight calves cloned from somatic cells of single adult Science, 282 (1998),pp. 2095-2098
[14]	Miyoshi, K., Rzucidlo, S.J., Pratt, S.L. et al. Improvements in cloning efficiencies may be possible by increasing uniformity in recipient oocytes and donor cells Biol. Reprod., 68 (2003),pp. 1079-1086
[15]	Reynier, P., May-Panloup, P., Chretien, M.F. et al. Mitochondrial DNA content affects the fertilizability of human oocytes Mol. Hum. Reprod., 7 (2001),pp. 425-429
[16]	Tamassia, M., Heyman, Y., Lavergne, Y. et al. Reproduction, 126 (2003),pp. 629-637
[17]	Taneja, M., French, R., Levine, H. et al. Clinical and pathological status of cloned calves born pre-term Theriogenology, 55 (2001),p. 293
[18]	Wells, D.N., Laible, G., Tucker, F.C. et al. Coordination between donor cell type and cell cycle stage improves nuclear cloning efficiency in cattle Theriogenology, 59 (2003),pp. 45-59
[19]	Yan, J.B., Wang, S., Huang, W.Y. et al. Transgenic mice can express mutant human coagulation factor IX with higher level of clotting activity Biochem. Genetics, 44 (2006),pp. 349-360
[20]	Yang, X.Y., Zhao, J.G., Li, H. et al. Anim. Reprod. Sci., 104 (2007),pp. 28-37
[21]	Yang, X.Y., Zhao, J.G., Li, H.W. et al. Theriogenology, 64 (2005),pp. 1263-1272
[22]	Zhou, Q., Boulanger, L., Renard, J.P. A simplified method for the reconstruction of fully competent mouse zygotes from adult somatic donor nuclei Cloning, 2 (2000),pp. 35-44