Assessment of genetic variation in tomato (Solanum lycopersicum L.) inbred lines using SSR molecular markers

Solomon Benor^{a, b, #},
Mengyu Zhang^{a, #},
Zhoufei Wang^a,
Hongsheng Zhang^{a
, ,}

a.
State Key Lab of Crop Genetics and Germplasm Enhancement, College of Agriculture, Nanjing Agricultural University, Nanjing 210095, China
b.
Department of Agrobiodiversity, Institute of Crop Science, University of Kassel, 34109 Kassel, Germany; and Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466 Gatersleben, Germany

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Corresponding author: E-mail address: hszhang@njau.edu.cn (Hongsheng Zhang)

These authors contributed equally to this work.

摘要

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Abstract: A study was conducted to determine the genetic diversity of 39 determinate and indeterminate tomato inbred lines collected from China, Japan, S. Korea, and USA. Using 35 SSR polymorphic markers, a total of 150 alleles were found with moderate levels of diversity, and a high number of unique alleles existing in these tomato lines. The mean number of alleles per locus was 4.3 and the average polymorphism information content (PIC) was 0.31. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) clustering at genetic similarity value of 0.85 grouped the inbred lines into four groups, where one USA cultivar formed a separate and more distant cluster. The most similar inbred lines are from USA, both with determinate type, whereas the most different lines are from USA (Us-16) and Japan (Ja-2) with determinate and indeterminate growth habit, respectively. Clustering was consistent with the known information regarding geographical location and growth habit. The genetic distance information reported in this study might be used by breeders when planning future crosses among these inbred lines.
- SSR markers /
- genetic diversity /
- growth characterization
These authors contributed equally to this work.
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