Differentiation potential of bone marrow mesenchymal stem cells in duck

Linfeng Li^{a, b},
Xiujuan Bai^b,
Xuelian Gong^a,
Hongkun Liu^a,
Lina Chen^a,
Weijun Guan^{a
, ,},
Yuehui Ma^{a
, ,}

a.
Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China
b.
College of Animal Science and Technology, Northeast Agricultural University, Harbin 150000, China

More Information

Corresponding author: E-mail address: wjguan86@126.com (Weijun Guan); E-mail address: yuehui.ma@263.net (Yuehui Ma)

摘要

- /
- /
- /
Abstract: The bone marrow mesenchymal stem cells (MSCs) are multipotent stem cells which can differentiate into mesenchymal cellsin vitro. In this study, MSCs in duck were isolated from bone marrow by density gradient centrifuge separation, purified and expanded in the medium. The primary MSCs were expanded for passages. The different-passage MSCs were induced to differentiate into osteoblasts and neuron-like cells. Karyotype analysis indicated that MSCs kept diploid condition and the hereditary feature was stable. The different-passage MSCs expressed CD44, ICAM- and SSEA-4, but not CD34, CD45 and SSEA-when detected by immunofluorescence staining. There was no significant difference among the positive rates of passages 2, 6 and 8 (P > 0.05), but a significant difference existed among those of passages 2, 6, 8 and 11 ( P < 0.05). After the osteogenic inducement was added, the induced different-passage MSCs expressed high-level alkaline phosphatase (ALP), and are positive for tetracycline staining, Alizarin Red staining and Von Kossa staining. After the neural inducement was added, about 70% cells exhibited typical neuron-like phenotype, the induced different-passage MSCs expressed Nestin, neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) when detected by immunofluorescence staining. There was no significant difference among the positive rates of passages 3, 4 and 6 ( P>0.05), but a significant difference existed among those of passages 3, 4, 6 and 8 (P<0.05). These results suggest that MSCs in duck were capable of differentiating into osteoblasts and neuron-like cellsin vitro.
- duck /
- mesenchymal stem cells /
- induction /
- differentiation

HTML全文

参考文献(13)

[1]	Azizi, S.A., Stokes, D., Augelli, B. et al. Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rat—similarities to astrocyte grafts Proc. Natl. Acad. Sci. USA, 95 (1998),pp. 3908-3913
[2]	Cheng, L., Qasba, P., Vanguri, P. et al. Human mesenchymal stem cells support megakaryocyte and pro-platelet formation from CD34(+) hematopoietic progenitor cells J. Cell Physiol., 184 (2000),pp. 58-69
[3]	Deans, R.J., Moseley, A.B. Mesenchymal stem cells: Biology and potential clinical uses Exp. Hematol., 28 (2000),pp. 875-884
[4]	Devine, S.M., Bartholomew, A.M., Mahmud, N. et al. Mesenchymal stem cells are capable of homing to the bone marrow of non-human primates following systemic infusion Exp. Hematol., 29 (2001),pp. 244-255
[5]	Hirofumi, S., Kentaro, Y., Kouichi, H. et al. Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage Biochem. Biophys. Res. Commun., 345 (2006),pp. 926-932
[6]	Ishii, K., Katayama, M., Hori, K. et al. Neurosci. Lett., 163 (1993),pp. 159-162
[7]	Kawarai, S., Hashizaki, K., Kitao, S. et al. Establishment and characterization of primary canine hepatocellular carcinoma cell lines producing alpha-fetoprotein Vet. Immunol. Immunopathol., 113 (2006),pp. 30-36
[8]	Majumdar, M.K., Thiede, M.A., Mosca, J.D. et al. Phenotypic and functional comparison of cultures of marrow-derived mesenchymal stem cells (MSCs) and stromal cells J. Cell Physiol., 176 (1998),pp. 57-66
[9]	Pereira, R.F., O'Hara, M.D., Laptev, A.V. et al. Marrow stromal cells as a source of progenitor cells for nonhematopoietic tissues in transgenic mice with a phenotype of osteogenesis imperfecta Proc. Natl. Acad. Sci. USA, 95 (1998),pp. 1142-1147
[10]	Pittenger, M.F., Mackay, A.M., Beck, S.C. et al. Multilineage potential of adult human mesenchymal stem cells Science, 284 (1999),pp. 143-147
[11]	Prockop, D.J. Marrow stromal cells as stem cells for nonhematopoietic tissues Science, 276 (1997),pp. 71-74
[12]	Reese, J.S., Koc, O.N., Gerson, S.L. Human mesenchymal stem cells provide stromal support for efficient CD34+ transduction J. Hematother. Stem Cell Res., 8 (1999),pp. 515-523
[13]	Woodbury, D., Schwarz, E.J., Prockop, D.J. et al. Adult rat and human bone marrow stromal cells differentiate into neurons J. Neurosci. Res., 61 (2000),pp. 364-370