Root and shoot traits responses to phosphorus deficiency and QTL analysis at seedling stage using introgression lines of rice

Key Laboratory of Crop Genomics and Genetic Improvement of Ministry of Agriculture and Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China

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Corresponding author: E-mail address: lizichao@cau.edu.cn (Zichao Li)

摘要

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Abstract: Phosphorous (P) deficiency is a major restraint factor for crop production and plants have developed several mechanisms to adapt to low P stress. In this study, a set of 271 introgression lines (ILs) were used to characterize the responses of seedlings to low P availability and to identify QTLs for root traits, biomass, and plant height under P-deficiency and P-sufficiency conditions. Plant height, total dry weight, shoot dry weight, and root number were inhibited under P-deficiency, whereas maximum root length (MRL) and root-shoot ratio (RS) were induced by P-deficiency stress. Relative MRL (RMRL, the ratio of MRL under P-deficiency to MRL under P-sufficiency condition) and relative RS (RRS) were used to evaluate P-deficiency tolerance at the seedling stage. A total of 24 additive QTLs and 29 pairs of epistatic QTLs were detected, but only qRN4 was detected in both conditions. This suggested that different mechanisms may exist in both P supply levels. QTLs for adaptive traits (RMRL, RRS, RRV, and RRDW) and qRN4 consistently expressed to increase trait stability may contribute to P-deficiency tolerance. Twelve intervals were cluster regions of QTLs for P-deficiency tolerance, and one QTL (qRRS8) showed pleiotropic effects on P-deficiency tolerance and drought tolerance. These interesting QTLs can be used in marker-assisted breeding through the target ILs.
- introgression lines /
- phosphorous deficiency tolerance /
- QTL mapping /
- rice /
- root traits

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参考文献(27)

[1]	Batjes, N.H. A world data set of derived soil properties by FAO UNESCO soil unit for global modeling Soil Use Manage., 13 (1997),pp. 9-16
[2]	Chaubey, C.N., Senadhira, D., Gregorio, G.B. Theor. Appl. Genet., 89 (1994),pp. 313-317
[3]	Dobermann, A., Fairhurst, T.
[4]	Eshed, Y., Zamir, D. Genetics, 141 (1995),pp. 1147-1162
[5]	Fageria, N.K., Morais, O.P., Baligar, V.C. et al. Response of rice cultivars to phosphorus supply on an oxisol Fert. Res., 16 (1988),pp. 195-206
[6]	He, Y., Lian, H., Yan, X. Localized supply of phosphorus induces root morphological and architectural changes of rice in split and stratified soil cultures Plant Soil, 248 (2003),pp. 247-256
[7]	Hou, X.L., Wu, P., Jiao, F.C. et al. Plant Cell Environ., 28 (2005),pp. 353-364
[8]	Ismail, A.M., Heuer, S., Thomson, M.J. et al. Genetic and genomic approaches to develop rice germplasm for problem soils Plant Mol. Biol., 65 (2007),pp. 547-570
[9]	Jones-Rhoades, M.W., Bartel, D.P. Computational identification of plant microRNAs and their targets, including a stress-induced miRNA Mol. Cell, 14 (2004),pp. 787-799
[10]	Li, Z.C., Mu, P., Li, C.P. et al. Theor. Appl. Genet., 110 (2005),pp. 1244-1252
[11]	Li, Z.K. QTL × environment interactions in rice. 1. Heading date and plant height Theor. Appl. Genet., 108 (2003),pp. 141-153
[12]	Liu, Y., Li, Z.C.M.G.H., Zhang, H.L. et al. Acta Agron. Sin., 31 (2005),pp. 238-242
[13]	Lynch, J. Root architecture and plant productivity Plant Physiol., 109 (1995),pp. 7-13
[14]	Ni, J.J., Wu, P., Senadhira, D. et al. Theor. Appl. Genet., 97 (1998),pp. 1361-1369
[15]	Paszkowski, U., Kroken, S., Roux, C. et al. Rice phosphate transporters include an evolutionarily divergent gene specifically activated in arbuscular mycorrhizal symbiosis Proc. Natl. Acad. Sci. USA, 99 (2002),pp. 13324-13329
[16]	Qu, Y.Y., Mu, P., Zhang, H.L. et al. Mapping QTLs of root morphological traits at different growth stages in rice Genetica, 133 (2008),pp. 187-200
[17]	Raghothama, K.G., Karthikeyan, A.S. Phosphate acquisition Plant Soil, 274 (2005),pp. 37-49
[18]	Shimizu, A., Kato, K., Komatsu, A. et al. Theor. Appl. Genet., 117 (2008),pp. 987-996
[19]	Shimizu, A., Yanagihara, S., Kawasaki, S. et al. Theor. Appl. Genet., 109 (2004),pp. 1361-1368
[20]	Wasaki, J., Yonetani, R., Shinano, T. et al. New Phytol., 158 (2003),pp. 239-248
[21]	Wissuwa, M., Ae, N. Plant Soil, 237 (2001),pp. 275-286
[22]	Wissuwa, M., Wegner, J., Ae, N. et al. Theor. Appl. Genet., 105 (2002),pp. 890-897
[23]	Wissuwa, M., Yano, M., Ae, N. Theor. Appl. Genet., 97 (1998),pp. 777-783
[24]	Wu, P., Ni, J.J. Detection of the quantitative trait loci with AFLP and RFLP markers for phosphorus uptake and use efficiency in rice J. Integr. Plant Biol., 42 (2000),pp. 229-233
[25]	Yang, J., Hu, C.C., Hu, H. et al. QTLNetwork: mapping and visualizing genetic architecture of complex traits in experimental populations Bioinformatics, 24 (2008),pp. 721-723
[26]	Yi, K.K., Wu, Z.C., Zhou, J. et al. Plant Physiol., 138 (2005),pp. 2087-2096
[27]	Yoshida, S., Forno, D.A., Cock, J.H. et al.