Effects of basic fibroblast growth factor on angiogenin expression and cell proliferation in H7402 human hepatoma cells

Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China

More Information

Corresponding author: E-mail address: wangli@nenu.edu.cn (Li Wang)

摘要

- /
- /
- /
Abstract: Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Basic fibroblast growth factor (bFGF), which is highly expressed in developing tissues and malignant cells, regulates cell growth, differentiation, and migration. Its expression is essential for the progression and metastasis of HCC. This study aims to investigate the effects of bFGF on the expression of angiogenin, another growth factor, which plays an important role in tumor angiogenesis, and on cell proliferation in H7402 human hepatoma cells. The bFGF sense cDNA or antisense cDNA was stably transfected into H7402 cells. Genomic DNA PCR analysis demonstrated that human bFGF sense cDNA or antisense cDNA was inserted into the genome. Furthermore, the expression of bFGF and angiogenin was examined by RT-PCR and Western blot assays. MTT and colony formation assays were employed to determine cell proliferation. Stable bFGF over-expressing and under-expressing transfectants were successfully established. Expression of angiogenin was decreased in the over-expressing bFGF cells (sense transfectants) and was increased in the under-expressing bFGF cells (antisense transfectants). Cell proliferation increased in the bFGF sense transfectants and decreased in the bFGF antisense transfectants. These results demonstrated that the endogenous bFGF may not only negatively regulate the angiogenin expression but also contribute to the overall cell proliferation in H7402 human hepatoma cells. This study may be helpful in finding a potential therapeutic approach to HCC.
- H7402 human hepatoma cells /
- basic fibroblast growth factor (bFGF) /
- angiogenin /
- cell proliferation

HTML全文

参考文献(32)

[1]	Arnaud, E., Touriol, C., Boutonnet, C. et al. A new 34-kilodalton isoform of human fibroblast growth factor 2 is cap dependently synthesized by using a non-AUG start codon and behaves as a survival factor Mol. Cell Biol., 19 (1999),pp. 505-514
[2]	Bikfalvi, A., Klein, S., Pintucci, G. et al. Biological roles of fibroblast growth factor-2 Endocr. Rev., 18 (1997),pp. 26-45
[3]	Bouche, G., Gas, N., Prats, H. et al. Basic fibroblast growth factor enters the nucleolus and stimulates the transcription of ribosomal genes in ABAE cells undergoing G0 → G1 transition Proc. Natl. Acad. Sci. USA, 84 (1987),pp. 6770-6774
[4]	Claus, P., Doring, F., Gringel, S. et al. Differential intranuclear localization of fibroblast growth factor-2 isoforms and specific interaction with the survival of motoneuron protein J. Biol. Chem., 278 (2003),pp. 479-485
[5]	Dailey, L., Ambrosetti, D., Mansukhani, A. et al. Mechanisms underlying differential responses to FGF signaling Cytokine Growth Factor Rev., 16 (2005),pp. 233-247
[6]	Delrieu, I. The high molecular weight isoforms of basic fibroblast growth factor (FGF-2): An insight into an intracrine mechanism FEBS Lett., 468 (2000),pp. 6-10
[7]	Fett, J.W., Strydom, D.J., Lobb, R.R. et al. Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cells Biochemistry, 24 (1985),pp. 5480-5486
[8]	Florkiewicz, R.Z., Anchin, J., Baird, A. J. Biol. Chem., 273 (1998),pp. 544-551
[9]	Hisai, H., Kato, J., Kobune, M. et al. Increased expression of angiogenin in hepatocellular carcinoma in correlation with tumor vascularity Clin. Cancer Res., 9 (2003),pp. 4852-4859
[10]	Kin, M., Sata, M., Ueno, T. et al. Basic fibroblast growth factor regulates proliferation and motility of human hepatoma cells by an autocrine mechanism J. Hepatol., 27 (1997),pp. 677-687
[11]	Kishimoto, K., Liu, S., Tsuji, T. et al. Endogenous angiogenin in endothelial cells is a general requirement for cell proliferation and angiogenesis Oncogene, 24 (2005),pp. 445-456
[12]	Liu, X., Zheng, S.X., Zhou, L.J. et al. Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes Acta Pharmacol. Sin., 21 (2000),pp. 19-22
[13]	Majumder, P.K., Yeh, J.J., George, D.J. et al. Prostate intraepithelial neoplasia induced by prostate restricted Akt activation: The MPAKT model Proc. Natl. Acad. Sci. USA, 100 (2003),pp. 7841-7846
[14]	Manoj, K.G., Qin, R.Y. Mechanism and its regulation of tumor-induced angiogenesis World J. Gastroenterol., 9 (2003),pp. 1144-1155
[15]	McKillop, I.H., Schrum, L.W. Alcohol and liver cancer Alcohol, 35 (2005),pp. 195-203
[16]	Mise, M., Arii, S., Higashituji, H. et al. Clinical significance of vascular endothelial growth factor and basic fibroblast growth factor gene expression in liver tumor Hepatology, 23 (1996),pp. 455-464
[17]	Musolino, C., Alonci, A., Bellomo, G. et al. Levels of soluble angiogenin in chronic myeloid malignancies: Clinical implications Eur. J. Haematol., 72 (2004),pp. 416-419
[18]	Olson, K.A., Fett, J.W., French, T.C. et al. Proc. Natl. Acad. Sci. USA, 92 (1995),pp. 442-446
[19]	Pederson, T. Growth factors in the nucleolus? J. Cell Biol., 143 (1998),pp. 279-281
[20]	Poon, R.T., Ng, I.O., Lau, C. et al. Correlation of serum basic fibroblast growth factor levels with clinicopathologic features and postoperative recurrence in hepatocellular carcinoma Am. J. Surg., 182 (2001),pp. 298-304
[21]	Rabie, A.B., Lu, M. Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor during healing of allogeneic bone graft Arch. Oral Biol., 49 (2004),pp. 1025-1033
[22]	Ramírez-Solis, R., Rivera-Pérez, J., Wallace, J.D. et al. Genomic DNA microextraction: A method to screen numerous samples Anal. Biochem., 201 (1992),pp. 331-335
[23]	Sheng, Z., Chang, S.B., Chirico, W.J. Expression and purification of a biologically active basic fibroblast growth factor fusion protein Protein Expr. Purif., 27 (2003),pp. 267-271
[24]	Sheng, Z., Lewis, J.A., Chirico, W.J. Nuclear and nucleolar localization of 18-kDa fibroblast growth factor-2 is controlled by C-terminal signals J. Biol. Chem., 279 (2004),pp. 40153-40160
[25]	Sheng, Z., Liang, Y., Lin, C.Y. et al. Direct regulation of rRNA transcription by fibroblast growth factor 2 Mol. Cell Biol., 25 (2005),pp. 9419-9426
[26]	Shimoyama, S., Kaminishi, M. Angiogenin in sera as an independent prognostic factor in gastric cancer J. Cancer Res. Clin. Oncol., 129 (2003),pp. 239-244
[27]	Sun, H.C., Tang, Z.Y. Angiogenesis in hepatocellular carcinoma: The retrospectives and perspectives J. Cancer Res. Clin. Oncol., 130 (2004),pp. 307-319
[28]	Tsuji, T., Sun, Y., Kishimoto, K. et al. Angiogenin is translocated to the nucleus of HeLa cells and is involved in ribosomal RNA transcription and cell proliferation Cancer Res., 65 (2005),pp. 1352-1360
[29]	Ugurel, S., Rappl, G., Tilgen, W. et al. Increased serum concentration of angiogenic factors in malignant melanoma patients correlates with tumor progression and survival J. Clin. Oncol., 19 (2001),pp. 577-583
[30]	Xu, Z.P., Tsuji, T., Riordan, J.F. et al. Identification and characterization of an angiogenin-binding DNA sequence that stimulates luciferase reporter gene expression Biochemistry, 42 (2003),pp. 121-128
[31]	Yasuda, E., Tokuda, H., Ishisaki, A. et al. PPAR-gamma ligands up-regulate basic fibroblast growth factor-induced VEGF release through amplifying SAPK/JNK activation in osteoblasts Biochem. Biophys. Res. Commun., 328 (2005),pp. 137-143
[32]	Yoshioka, N., Wang, L., Kishimoto, K. et al. A therapeutic target for prostate cancer based on angiogenin-stimulated angiogenesis and cancer cell proliferation Proc. Natl. Acad. Sci. USA, 103 (2006),pp. 14519-14524