Characterization of the promoter of phosphate transporter TaPHT1.2 differentially expressed in wheat varieties

Jun Miao^{a, b},
Jinghan Sun^a,
Dongcheng Liu^a,
Bin Li^a,
Aimin Zhang^a,
Zhensheng Li^{a
, ,},
Yiping Tong^{a
, ,}

a.
The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
b.
Vegetable Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China

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Corresponding author: E-mail address: zsli@genetics.ac.cn (Zhensheng Li); E-mail address: yptong@genetics.ac.cn (Yiping Tong)

摘要

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Abstract: TaPHT1.2 is a functional, root predominantly expressed and low phosphate (Pi) inducible high-affinity Pi transporter in wheat, whichis more abundant in the roots of P-efficient wheat genotypes (e.g., Xiaoyan 54) than in P-inefficient genotypes (e.g., Jing 411) under bothPi-deficient and Pi-sufficient conditions. To characterize TaPHT1.2 further, we genetically mapped a TaPHT1.2 transporter, TaPHT1.2-D1, on the long arm of chromosome 4D using a recombinant inbred line population derived from Xiaoyan 54 and Jing 411, and isolated a1,302 bp fragment of the TaPHT1.2-D1 promoter (PrTaPHT1.2-D1) from Xiaoyan 54. TaPHT1.2-D1 shows collinearity with OsPHT1.2 that has previously been reported to mediatethe translocation of Pi from roots to shoots.PrTaPHT1.2-D contains a number of Pi-starvation responsive elements, including P1BS, WRKY-binding W-box, and helix-loop-helix-binding elements. PrTaPHT1.2-D1 wasthen used to drive expression of β-glucuronidase (GUS) reporter gene in Arabidopsis through Agrobacterium-mediated transformation. Histochemical analysis of transgenic Arabidopsis plants showed that the reporter gene was specifically induced by Pi-starvation and predominantly expressed in the roots. As there is only one SNP between the TaPHT1.2-D1 promoters of Xiaoyan 54 and Jing 411, and thisSNP does not exist within the Pi-starvation responsive elements, the differential expression of TaPHT1.2 in Xiaoyan 54 and Jing 411 maynot be caused by this SNP.
- high-affinity phosphate transporter /
- promoter /
- differential expression

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参考文献(45)

[1]	Ai, P., Sun, S., Zhao, J. et al. Plant J., 57 (2009),pp. 798-809
[2]	Benbouza, H., Jacquemin, J.M., Baudoin, J.P. et al. Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels Biotech. Agron. Soc. Environ., 10 (2006),pp. 77-81
[3]	Burleigh, S.H., Harrison, M.J. Plant Mol. Biol., 34 (1997),pp. 199-208
[4]	Clough, S.J., Steven, J., Bent, A.F. Plant J., 16 (1998),pp. 735-743
[5]	Davies, T.G.E., Ying, J., Xu, Q. et al. Expression analysis of putative high-affinity phosphate transporters in Chinese winter wheats Plant Cell Environ., 25 (2002),pp. 1325-1340
[6]	Devaiah, B.N., Karthikeyan, A.S., Raghothama, K.G. Plant Physiol., 143 (2007),pp. 1789-1801
[7]	Elmayan, T., Tepfer, M. Transgenic Res., 4 (1995),pp. 388-396
[8]	Franco-Zorrilla, J.M., Martin, A.C., Leyva, A. et al. Plant Physiol., 138 (2005),pp. 847-857
[9]	Franco-Zorrilla, J.M., Valli, A., Todesco, M. et al. Target mimicry provides a new mechanism for regulation of microRNA activity Nat. Genet., 39 (2007),pp. 1033-1037
[10]	Gordon-Weeks, R., Tong, Y.P., Davies, T.G. et al. Restricted spatial expression of a high-affinity phosphate transporter in potato roots J. Cell Sci., 116 (2003),pp. 3135-3144
[11]	Hammond, J.P., Bennett, M.J., Bowen, H.C. et al. Plant Physiol., 132 (2003),pp. 578-596
[12]	Hammond, J.P., Broadley, M.R., White, P.J. Genetic responses to phosphorus deficiency Ann. Bot., 94 (2004),pp. 323-332
[13]	Hammond, J.P., White, P.J. Sucrose transport in the phloem: Integrating root responses to phosphorus starvation J. Exp. Bot., 59 (2008),pp. 93-109
[14]	Hou, X.L., Wu, P., Jiao, F.C. et al. Plant Cell Environ., 28 (2005),pp. 353-364
[15]	Karthikeyan, A.S., Varadarajan, D.K., Jain, A. et al. Planta, 225 (2007),pp. 907-918
[16]	Koyama, T., Ono, T., Shimizu, M. et al. J. Biosci. Bioeng., 99 (2005),pp. 38-42
[17]	Lander, E.S., Botstein, D. Mapping mendelian factors underlying quantitative traits using RFLP linkage maps Genetics, 121 (1989),pp. 185-199
[18]	Li, Y.L., Tong, Y.P., Li, B. et al. Acta Bot. Boreal.-Occident. Sin., 28 (2008),pp. 1303-1307
[19]	Liu, C., Muchhal, U.S., Uthappa, M. et al. Tomato phosphate transporter genes are differentially regulated in plant tissues by phosphorus Plant Physiol., 116 (1998),pp. 91-99
[20]	Martín, A.C., del Pozo, J.C., Iglesias, J. et al. Plant J, 24 (2000),pp. 559-567
[21]	Miftahudin, Ross, K., Ma, X.F., Mahmoud, A.A. et al. Analysis of expressed sequence tag loci on wheat chromosome group 4 Genetics, 168 (2004),pp. 651-663
[22]	Mudge, S.R., Rae, A.L., Diatloff, E. et al. Plant J., 31 (2002),pp. 341-353
[23]	Nagy, R., Vasconcelos, M.J., Zhao, S. et al. Plant Biology (Stuttgart, Germany), 8 (2006),pp. 186-197
[24]	Okamura, H., Hanaoka, S., Nagadoi, A. et al. J. Mol. Biol., 295 (2000),pp. 1225-1236
[25]	Paszkowski, U., Kroken, S., Roux, C. et al. Rice phosphate transporters include an evolutionarily divergent gene specifically activated in arbuscular mycorrhizal symbiosis Proc. Natl. Acad. Sci. USA, 99 (2002),pp. 13324-13329
[26]	Rae, A.L., Cybinski, D.H., Jarmey, J.M. et al. Characterization of two phosphate transporters from barley; evidence for diverse function and kinetic properties amongst members of the Pht1 family Plant Mol. Biol., 53 (2003),pp. 27-36
[27]	Raghothama, K.G., Karthikeyan, A.S. Phosphate acquisition Plant Soil, 274 (2005),pp. 37-49
[28]	Raghothama, K.G. Phosphate acquisition Annu. Rev. Plant Physiol. Plant Mol. Biol., 50 (1999),pp. 665-693
[29]	Rubio, V., Linhares, F., Solano, R. et al. A conserved MYB transcription factor involved in phosphate starvation signaling both in vascular plants and in unicellular algae Genes Dev., 15 (2001),pp. 2122-2133
[30]	Schachtman, D.P., Reid, R.J., Ayling, S.M. Phosphorus uptake by plants: From soil to cell Plant Physiol., 116 (1998),pp. 447-453
[31]	Schünmann, P.H., Richardson, A.E., Smith, F.W. et al. J. Exp. Bot., 55 (2004),pp. 855-865
[32]	Schünmann, P.H., Richardson, A.E., Vickers, C.E. et al. Plant Physiol., 136 (2004),pp. 4205-4214
[33]	Shin, H., Shin, H.S., Dewbre, G.R. et al. Plant J., 39 (2004),pp. 629-642
[34]	Slabaugh, M., Huestis, G., Leonard, J. et al. Sequence based genetic markers for genes and gene families: Single strand conformational polymorphisms for the fatty acid synthesis genes of Cuphea Theor. Appl. Genet., 94 (1997),pp. 400-408
[35]	Smith, F.W., Cybinski, D., Rae, A.E.
[36]	Smith, F.W., Jackson, W.A. Plant Physiol., 84 (1987),pp. 1314-1318
[37]	Smith, F.W., Jackson, W.A. Plant Physiol., 84 (1987),pp. 1319-1324
[38]	Smith, F.W., Mudge, S.R., Rae, A.L. et al. Phosphate transport in plants Plant Soil, 248 (2003),pp. 71-83
[39]	Stomp, A.M.
[40]	Tittarelli, A., Milla, L., Vargas, F. et al. J. Exp. Bot., 58 (2007),pp. 2573-2582
[41]	Vance, C.P., Uhde-Stone, C., Allan, D.L. Phosphorus acquisition and use: Critical adaptations by plants for securing a non-renewable resource New Phytologist, 157 (2003),pp. 423-447
[42]	Varscht, J., Tomos, D., Komor, E. Planta, 224 (2006),pp. 1303-1314
[43]	Wang, Y.H., Garvin, D.F., Kochian, L.V. Nitrate-induced genes in tomato roots: Array analysis reveals novel genes that may play a role in nitrogen nutrition Plant Physiol., 127 (2001),pp. 345-359
[44]	Zeng, Y.J., Ying, J., Liu, J.Z. et al. Function analysis of a wheat phosphate transporter in yeast mutant Acta Genetica Sinica, 29 (2002),pp. 1017-1020
[45]	Zhou, J., Jiao, F.C., Wu, Z.C. et al. Plant Physiol., 146 (2008),pp. 1673-1686

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doi: 10.1016/S1673-8527(08)60135-6

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Characterization of the promoter of phosphate transporter TaPHT1.2 differentially expressed in wheat varieties

Corresponding author: E-mail address: zsli@genetics.ac.cn (Zhensheng Li); E-mail address: yptong@genetics.ac.cn (Yiping Tong)

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doi: 10.1016/S1673-8527(08)60135-6

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Characterization of the promoter of phosphate transporter TaPHT1.2 differentially expressed in wheat varieties

Corresponding author: E-mail address: zsli@genetics.ac.cn (Zhensheng Li); E-mail address: yptong@genetics.ac.cn (Yiping Tong)

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