Genetic analysis and molecular mapping of a novel gene for zebra mutation in rice (Oryza sativa L.)

Key Laboratory of Biotechnology and Crop Quality Improvement, Ministry of Agriculture; Rice Research Institute, Southwest University, Chongqing 400716, China

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Corresponding author: E-mail address: hegh1968@yahoo.com.cn (Guanghua He)

摘要

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Abstract: A novel zebra mutant, zebra-15, derived from the restorer line Jinhui10 (Oryza sativa L. ssp. indica) treated by EMS, displayed a distinctive zebra leaf from seedling stage to jointing stage. Its chlorophyll content decreased (55.4%) and the ratio of Chla/Chlb increased (90.2%) significantly in the yellow part of the zebra-15, compared with the wild type. Net photosynthetic rate and fluorescence kinetic parameters showed that the decrease of chlorophyll content significantly influenced the photosynthetic efficiency of the mutant. Genetic analysis of F₂ segregation populations derived from the cross of Xinong1A and zebra-15 indicated that the zebra leaf trait is controlled by a single recessive nuclear gene. Ninety-eight out of four hundred and eighty pairs of SSR markers showed the diversity between the Xinong1A and the zebra-15, their F₂ population was then used for gene mapping. Zebra-15 (Z-15) gene was primarily restricted on the short arm of chromosome 5 by 150 F₂ recessive individuals, 19.6 cM from marker RM3322 and 6.0 cM from marker RM6082. Thirty-six SSR markers were newly designed in the restricted location, and the Z-15 was finally located between markers nSSR516 and nSSR502 with the physical region 258 kb by using 1,054 F₂ recessive individuals.
- molecular mapping /
- zebra mutant /
- chlorophyll

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参考文献(34)

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