Genetic analysis and fine-mapping of a dwarfing with withered leaf-tip mutant in rice

Liang Jiang^{a, b},
Longbiao Guo^{a
, ,},
Hua Jiang^a,
Dali Zeng^a,
Jiang Hu^a,
Liwen Wu^{a, b},
Jian Liu^a,
Zhenyu Gao^a,
Qian Qian^{a
, ,}

a.
State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
b.
Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China

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Corresponding author: E-mail address: guolongb@mail.hz.zj.cn (Longbiao Guo); E-mail address: qianqian188@hotmail.com (Qian Qian)

摘要

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Abstract: A dwarf mutant of rice (Oryza sativa L.) by mutagenesis of ethylene methylsulfonate (EMS) treatment from Nipponbare was identified. The mutant exhibited phenotypes of dwarfism and withered leaf tip (dwl1). Based on the internode length of dwl1, this mutant belongs to the dm type of dwarfing. Analysis of elongation of the second sheath and α-amylase activity in endosperm showed that the phenotype caused by dwl1 was insensitive to gibberellin acid treatment. Using a large F₂ population derived from a cross between the dwl1 and an indica rice variety, TN1, the DWL1 gene was mapped to the terminal region of the long arm of chromosome 3. Fine-mapping delimited it into a 46 kb physical distance between two STS markers, HL921 and HL944, where 6 open reading frames were predicted. Cloning of DWL1 will contribute to dissecting molecular mechanism that regulates plant height in rice, which will be beneficial to molecular assisted selection of this important trait.
- mutant /
- dwarf /
- fine mapping /
- rice

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[1]	Akagi, H., Yokozeki, Y., Inagaki, A. et al. Micron a microsatellite-targeting transposable element in the rice genome Mol. Genet. Genomics, 266 (2001),pp. 471-480
[2]	Ashikari, M., Wu, J., Yano, M. et al. Proc. Natl. Acad. Sci. USA, 96 (1999),pp. 10284-10289
[3]	Azpiroz, R., Wu, Y., LoCascio, J.C. et al. Plant Cell, 10 (1998),pp. 219-230
[4]	Borlaug, N.E. Contributions of conventional plant breeding to food production Science, 219 (1983),pp. 689-693
[5]	Chaffey, N., Cholewa, E., Regan, S. et al. Physiol. Plant, 114 (2002),pp. 594-600
[6]	Donld, C.M. The breeding of crop ideotypes Euphytica, 17 (1968),pp. 385-403
[7]	Evans, L.T.
[8]	Gale, M.D., Youssefian, S.
[9]	Gomi, K., Sasaki, A., Itoh, H. et al. GID2, an F-box subunit of the SCF E3 complex, specifically interacts with phosphorylated SLR1 protein and regulates the gibberellin-dependent degradation of SLR1 in rice Plant J., 37 (2004),pp. 626-634
[10]	Guo, L.B., Chu, C.C., Qian, Q. Rice mutants and functional genomics Chin. Bull. Bot., 23 (2006),pp. 1-13
[11]	Guo, L.B., Xing, Y.Z., Mei, H.W. et al. Dissection of component QTL expression in yield formation in rice Plant Breed., 124 (2005),pp. 127-132
[12]	Guo, Y., Yuan, Z., Sun, Y. et al. Acta. Bot. Sin., 46 (2004),pp. 846-853
[13]	Hong, Z., Ueguchi-Tanaka, M., Fujioka, S. et al. Plant Cell, 17 (2005),pp. 2243-2254
[14]	Hooley, R. Gibberellins: Perception, transduction and responses Plant Mol. Biol., 26 (1994),pp. 1529-1555
[15]	Ikeda, A., Ueguchi-Tanaka, M., Sonoda, Y. et al. Plant Cell, 13 (2001),pp. 999-1010
[16]	Itoh, H., Tatsumi, T., Sakamoto, T. et al. Plant Mol. Biol., 54 (2004),pp. 533-547
[17]	Itoh, H., Ueguchi-Tanaka, M., Sentoku, N. et al. Cloning and functional analysis of two gibberellin 3 beta -hydroxylase genes that are differently expressed during the growth of rice Proc. Natl. Acad. Sci. USA, 98 (2001),pp. 8909-8914
[18]	Jiang, H., Guo, L.B., Qian, Q. Recent progress on rice genetics in China J. Integr. Plant Biol., 49 (2007),pp. 776-790
[19]	Jiang, H., Guo, L.B., Xue, D.W. et al. Genetic analysis and genemapping of two reduced-culm-number mutants in rice J. Integr. Plant Biol., 48 (2006),pp. 341-347
[20]	Khush, G.S.
[21]	Kinoshita, T. Report of the committee on gene symbolization, nomenclature and linkage groups Rice Genet. Newslett., 12 (1995),pp. 9-115
[22]	Lander, E.S., Green, P., Abrahamson, J. et al. Mapmaker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations Genomics, 1 (1987),pp. 174-181
[23]	Li, J., Chory, J. A putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction Cell, 90 (1997),pp. 929-938
[24]	Li, X.Y., Qian, Q., Fu, Z. et al. Control of tillering in rice Nature, 422 (2003),pp. 618-621
[25]	McCouch, S.R., Teytelman, L., Xu, Y. et al. DNA Res., 9 (2002),pp. 257-279
[26]	Mitsunaga, S., Tashira, T., Yamaguchi, J. Identification and characterization of gibberellin insensitive mutants selected from among dwarf mutants of rice Theor. Appl. Genet., 87 (1994),pp. 705-712
[27]	Mori, M., Nomura, T., Ooka, H. et al. Isolation and characterization of a rice dwarf mutant with a defect in brassinosteroid biosynthesis Plant Physiol., 130 (2002),pp. 1152-1161
[28]	Multani, D.S., Briggs, S.P., Chamberlin, M.A. et al. Science, 302 (2003),pp. 81-84
[29]	Murakami, Y. A new rice seedling test for gibberellins, “microdrop method” and its use for testing extracts of rice and morning glory Bot. Mag., 81 (1968),pp. 33-43
[30]	Murray, M.G., Thompson, W.F. Rapid isolation of high molecular weight plant DNA Nucleic Acids Res., 8 (1980),pp. 4321-4325
[31]	Ogi, Y., Kato, H., Maruyama, K. et al. Japan J. Breed, 48 (1993),pp. 267-275
[32]	Piepho, H.P. A simple procedure for yield component analysis Euphytica, 84 (1995),pp. 43-48
[33]	Qian, Q., Cheng, S.H.
[34]	Reiter, W.D., Chapple, C.C.S., Somerville, C.R. Science, 261 (1993),pp. 1032-1035
[35]	Ross, J.J., Murfet, I.C., Reid, J.B. Gibberellin mutants Physiol. Plant, 100 (1997),pp. 550-560
[36]	Ruellanda, E., Campalansa, A., Selman-Houseinb, G. et al. Cellular and subcellular localization of the lignin biosynthetic enzymes caffeic acid-o-methyltransferase, cinnamylalcohol dehydrogenase and cinnamoyl-coenzyme A reductase in two monocots, sugarcane and maize Physiol. Plant, 117 (2003),pp. 93-99
[37]	Shomura, A., Izawa, T., Ebana, K. et al. Deletion in a gene associated with grain size increased yields during rice domestication Nat. Genet., 40 (2008),pp. 1023-1028
[38]	Song, X.J., Huang, W., Shi, M. et al. A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase Nat. Genet., 39 (2007),pp. 623-630
[39]	Takahashi, T., Gasch, A., Nishizawa, N. et al. Genes Dev., 9 (1995),pp. 97-107
[40]	Takeda, K. Internode elongation and dwarfism in some gramineous plants Gamma Field Sym., 16 (1997),pp. 1-18
[41]	Tanabe, S., Ashikari, M., Fujisawa, Y. et al. Plant Cell, 17 (2005),pp. 776-790
[42]	Temnykh, S., William, D.P., Ayres, N. et al. Mapping and genome organization of microsatellite sequences in rice Theor. Appl. Genet., 100 (2000),pp. 697-712
[43]	Wu, P. Analysis of the genetic relativity between plant height and tillering of rice using the RFLP markers Sci. China (Ser C), 26 (1996),pp. 264-270
[44]	Xue, W., Xing, Y., Weng, X. et al. Nat. Genet., 40 (2008),pp. 761-767

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doi: 10.1016/S1673-8527(08)60226-X

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Genetic analysis and fine-mapping of a dwarfing with withered leaf-tip mutant in rice

Corresponding author: E-mail address: guolongb@mail.hz.zj.cn (Longbiao Guo); E-mail address: qianqian188@hotmail.com (Qian Qian)

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doi: 10.1016/S1673-8527(08)60226-X

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Genetic analysis and fine-mapping of a dwarfing with withered leaf-tip mutant in rice

Corresponding author: E-mail address: guolongb@mail.hz.zj.cn (Longbiao Guo); E-mail address: qianqian188@hotmail.com (Qian Qian)

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