Two sequence alterations, a 136 bp InDel and an A/C polymorphic site, in the S5 locus are associated with spikelet fertility of indica-japonica hybrid in rice

Qing Ji^{a, #},
Jufei Lu^{b, #},
Qing Chao^a,
Yan Zhang^a,
Meijing Zhang^a,
Minghong Gu^{b
, ,},
Mingliang Xu^{a, c
, ,}

a.
National Maize Improvement Center, China Agricultural University, Beijing 100193, China
b.
Key lab for plant biotechnology of Jiangsu province, Yangzhou University, Yangzhou 225009, China
c.
Key Laboratory of Crop Genetic Improvement, Beijing 100193, China

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Corresponding author: E-mail address: gumh@yzu.edu.cn (Minghong Gu); E-mail address: mxu@cau.edu.cn (Mingliang Xu)

These authors contributed equally to this work.

摘要

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Abstract: The rice indica/japonica hybrid shows strong heterosis. However, such inter-subspecific hybrid can't be directly used in rice production due to its low spikelet fertility. The S5 locus was proved to be associated with fertility of indica/japonica hybrid and its S5n allele from wide-compatibility variety (WCV) is capable to overcome fertility barrier. In the present study, we reported the causal sites in the S5 locus responsible for compatibility of indica/japonica hybrid. Fine-mapping of the S5 locus using the 11 test-cross families pinpoints a candidate S5 locus encoding aspartic protease (Asp). Intragenic recombination within the Asp gene happened in a number of recombinants, resulting in chimeric S5j-S5n alleles. Just like S5n, the chimeric S5j-S5n allele displayed higher spikelet fertility when combined with the S5i allele. In the complementary test, however, the S5n allele from WCVs failed to enhance fertilities of the indica/japonica hybrids. Compared to both indica and japonica varieties, all nine WCVs from different resources are characterized with a 136 bp deletion in the Asp N-terminus, which probably renders the S5n allele non-functional. Furthermore, an A/C polymorphic site is detected 1,233 bp downstream of the Asp start codon. The heterozygous A/C site of the Asp gene in indica/japonica hybrid is believed to be the casual factor to cause partial sterility. The functional makers based on the two polymorphic sites will be broadly used in developing wide-compatibility rice varieties.
- rice /
- wide-compatibility
These authors contributed equally to this work.
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doi: 10.1016/S1673-8527(09)60025-4

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Two sequence alterations, a 136 bp InDel and an A/C polymorphic site, in the S5 locus are associated with spikelet fertility of indica-japonica hybrid in rice

Corresponding author: E-mail address: gumh@yzu.edu.cn (Minghong Gu); E-mail address: mxu@cau.edu.cn (Mingliang Xu)

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doi: 10.1016/S1673-8527(09)60025-4

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Two sequence alterations, a 136 bp InDel and an A/C polymorphic site, in the S5 locus are associated with spikelet fertility of indica-japonica hybrid in rice

Corresponding author: E-mail address: gumh@yzu.edu.cn (Minghong Gu); E-mail address: mxu@cau.edu.cn (Mingliang Xu)

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