Characterization of seedling proteomes and development of markers to distinguish the Brassica A and C genomes

a.
College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China
b.
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China
c.
Institute for Plant Breeding and Crop Science I, Justus Liebig University, Heinrich-Buff-Ring 26-32, Giessen 35392, Germany

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Corresponding author: E-mail address: wangyp@yzu.edu.cn (Youping Wang)

摘要

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Abstract: The diploid species Brassica rapa (genome AA) and B. oleracea (genome CC) were compared by full-scale proteome analyses of seedling. A total of 28.2% of the proteins was common to both species, indicating the existence of a basal or ubiquitous proteome. However, a number of discriminating proteins (32.0%) and specific proteins (39.8%) of the Brassica A and C genomes, respectively, were identified, which could represent potentially species-specific functions. Based on these A or C genome-specific proteins, a number of PCR-based markers to distinguish B. rapa and B. oleracea species were also developed.
- Brassica rapa /
- Brassica oleracea /
- proteomics /
- molecular marker

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