Polymorphisms in PPARD, PPARG and APM1 associated with four types of Traditional Chinese Medicine constitutions

Yanrui Wu^{a, #},
Yina Cun^{a, a, #},
Jing Dong^b,
Jingru Shao^a,
Shengjun Luo^a,
Shengjie Nie^a,
Haijing Yu^a,
Bingrong Zheng^a,
Qi Wang^{c
, ,},
Chunjie Xiao^{a
, ,}

a.
Human Genetics Center of Yunnan University, Kunming 650091, China
b.
The General Hospital of the Air Force, Beijing 100036, China
c.
Center for Studies in Constitution Research of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China

More Information

Corresponding author: E-mail address: wangqi710@126.com (Qi Wang); E-mail address: cjxiao2009@gmail.com (Chunjie Xiao)

These authors contributed equally to this work.

摘要

- /
- /
- /
Abstract: Based on the theory of constitution of Traditional Chinese Medicine (TCM), the human population is divided into nine constitutions including one balanced constitution (Normality) and eight unbalanced constitutions (Yang-deficiency, Yin-deficiency, Phlegm-wetness, Qi-deficiency, Wetness-heat, Blood stasis, Depressed constitution, and Inherited special constitution). Different constitutions have specific metabolic features and different susceptibility to certain diseases. However, whether a genetic basis accounts for such constitution classification is yet to be determined. Here we performed a genetic study to assess the association between genetic variations of metabolic genes including PPARD, PPARG and APM1 and the constitutions. A total of 233 individuals of the Han population in China were classified into four groups, Normality, Yang-deficiency, Yin-deficiency and Phlegm-wetness with whom 23 single nucleotide polymorphisms (SNPs) in the three genes were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Biased distribution ofPPARD rs2267669 and rs2076167, APM1 rs7627128 and rs1063539 in Yang-deficiency, PPARG Pro12Ala in Yin-deficiency and PPARD rs2076167, APM1 rs266729 and rs7627128 in Phlegm-wetness were observed. The frequencies of Haplotype13 (Hap13) of PPARG in Yin-deficiency, Hap25 of APM1 in Yang-deficiency and Hap2 of PPARD and Hap14 of PPARG in Phlegm-wetness, were significantly different from those in Normality, suggesting those might be group-associated haplotypes. These results suggested that single SNP and haplotypes of PPARD, PPARG and APM1 may underlie the genetic basis of the constitutions classified in TCM.
- Traditional Chinese Medicine /
- constitution /
- classification /
- SNP
These authors contributed equally to this work.
注释:

HTML全文

参考文献(32)

[1]	Charo, I.F. Macrophage polarization and insulin resistance: PPARgamma in control Cell Metab., 6 (2007),pp. 96-98
[2]	Chen, S., Lv, F., Gao, J. et al. HLA class II polymorphisms associated with the physiologic characteristics defined by Traditional Chinese Medicine: linking modern genetics with an ancient medicine J. Altern. Complement. Med., 13 (2007),pp. 231-239
[3]	Dong, J., Wang, Q., Wang, D. et al. The analysis of metabolic sydrome in terms of Phlegwetness Journal of Beijing University of Traditional Chinese Medicine, 12 (2006),pp. 802-803
[4]	Franks, P.W., Jablonski, K.A., Delahanty, L. et al. The Pro12Ala variant at the peroxisome proliferator-activated receptor gamma gene and change in obesity-related traits in the Diabetes Prevention Program Diabetologia, 50 (2007),pp. 2451-2460
[5]	Gabriel, S.B., Schaffner, S.F., Nguyen, H. et al. The structure of haplotype blocks in the human genome Science, 296 (2002),pp. 2225-2229
[6]	Gross-Bellard, M., Oudet, P., Chambon, P. Isolation of high-molecular-weight DNA from mammalian cells Eur. J. Biochem., 36 (1973),pp. 32-38
[7]	Hesketh, T., Zhu, W.X. Health in China. Traditional Chinese medicine: one country, two systems BMJ, 315 (1997),pp. 115-117
[8]	Jiang, Y., Ma, Y., Yang, Y. et al. Discussion on diabetes in terms of Yang Deficiency Medicine World (2006),p. 114
[9]	Kang, E.S., Park, S.Y., Kim, H.J. et al. The influence of adiponectin gene polymorphism on the rosiglitazone response in patients with type 2 diabetes Diabetes Care, 28 (2005),pp. 1139-1144
[10]	Kersten, S., Desvergne, B., Wahli, W. Roles of PPARs in health and disease Nature, 405 (2000),pp. 421-424
[11]	Kyriakou, T., Collins, L.J., Spencer-Jones, N.J. et al. Adiponectin gene ADIPOQ SNP associations with serum adiponectin in two female populations and effects of SNPs on promoter activity J. Hum. Genet., 53 (2008),pp. 718-727
[12]	Lang, R. The susceptibility of Yin-deficiency constitution to diseases and its early intervention New Journal of Traditional Chinese Medicine, 36 (2004),pp. 70-71
[13]	Lee, C.H., Olson, P., Evans, R.M. Minireview: lipid metabolism, metabolic diseases, and peroxisome proliferator-activated receptors Endocrinology, 144 (2003),pp. 2201-2207
[14]	Lehrke, M., Lazar, M.A. The many faces of PPARgamma Cell, 123 (2005),pp. 993-999
[15]	Li, D., Tian, J., Wang, S. et al. Study review and prospect on inherent essence of Yang Deficiency Modern Journal of Integrated Traditional Chinese and Western Medicine, 9 (2000),pp. 1213-1215
[16]	Li, S., Li, L., Li, K. et al. Clin. Endocrinol., 68 (2008),pp. 885-889
[17]	Matsuzawa, Y., Funahashi, T., Kihara, S. et al. Adiponectin and metabolic syndrome Arterioscler. Thromb. Vasc. Biol., 24 (2004),pp. 29-33
[18]	Pajvani, U.B., Du, X., Combs, T.P. et al. Structure-function studies of the adipocyte-secreted hormone Acrp30/adiponectin. Implications fpr metabolic regulation and bioactivity J. Biol. Chem., 278 (2003),pp. 9073-9085
[19]	Qi, L., Doria, A., Manson, J.E. et al. Adiponectin genetic variability, plasma adiponectin, and cardiovascular risk in patients with type 2 diabetes Diabetes, 55 (2006),pp. 1512-1516
[20]	Qi, L., Li, T., Rimm, E. et al. Diabetes, 54 (2005),pp. 1607-1610
[21]	Rioux, J.D., Daly, M.J., Silverberg, M.S. et al. Genetic variation in the 5q31 cytokine gene cluster confers susceptibility to Crohn disease Nat. Genet., 29 (2001),pp. 223-228
[22]	Scherer, P.E. Adipose tissue: from lipid storage compartment to endocrine organ Diabetes, 55 (2006),pp. 1537-1545
[23]	Stumvoll, M., Wahl, H.G., Loblein, K. et al. Pro12Ala polymorphism in the peroxisome proliferator-activated receptor-gamma2 gene is associated with increased antilipolytic insulin sensitivity Diabetes, 50 (2001),pp. 876-881
[24]	Tschritter, O., Fritsche, A., Stefan, N. et al. Increased insulin clearance in peroxisome proliferator-activated receptor gamma2 Pro12Ala Metabolism, 52 (2003),pp. 778-783
[25]	Vaccaro, O., Lapice, E., Monticelli, A. et al. Pro12Ala polymorphism of the PPARgamma2 locus modulates the relationship between energy intake and body weight in type 2 diabetic patients Diabetes Care, 30 (2007),pp. 1156-1161
[26]	Vanttinen, M., Nuutila, P., Kuulasmaa, T. et al. Single nucleotide polymorphisms in the peroxisome proliferator-activated receptor delta gene are associated with skeletal muscle glucose uptake Diabetes, 54 (2005),pp. 3587-3591
[27]	Vidal-Puig, A.J., Considine, R.V., Jimenez-Linan, M. et al. Peroxisome proliferator-activated receptor gene expression in human tissues. Effects of obesity, weight loss, and regulation by insulin and glucocorticoids J. Clin. Invest., 99 (1997),pp. 2416-2422
[28]	Wang, Q. Classification and diagnosis basis of nine basic constitutions in Chinese medicine Journal of Beijing University of Traditional Chinese Medicine, 28 (2005),pp. 1-8
[29]	Wang, Q., Yao, S. Molecular basis for cold-intolerant yang-deficient constitution of traditional Chinese medicine Am. J. Chin. Med., 36 (2008),pp. 827-834
[30]	Wang, Q., Gong, H., Gao, J. Study on Characteristics of peripheral blood gene expression profile in the obesity with Phlegm-Dampness constitution Journal of Traditional Chinese Medicine, 47 (2006),pp. 851-858
[31]	Wang, Y.X., Lee, C.H., Tiep, S. et al. Peroxisome-proliferator-activated receptor delta activates fat metabolism to prevent obesity Cell, 113 (2003),pp. 159-170
[32]	Whitehead, J.P., Richards, A.A., Hickman, I.J. et al. Adiponectin—a key adipokine in the metabolic syndrome Diabetes. Obes. Metab., 8 (2006),pp. 264-280

施引文献

资源附件(0)

访问统计

点击查看大图

计量

文章访问数: 88
HTML全文浏览量: 28
PDF下载量: 1
被引次数: 0

姓名
邮箱
手机号码
标题
留言内容
验证码

留言板

doi: 10.1016/S1673-8527(09)60055-2

计量

Polymorphisms in PPARD, PPARG and APM1 associated with four types of Traditional Chinese Medicine constitutions

Corresponding author: E-mail address: wangqi710@126.com (Qi Wang); E-mail address: cjxiao2009@gmail.com (Chunjie Xiao)

计量

目录

留言板

doi: 10.1016/S1673-8527(09)60055-2

计量

出版历程

Polymorphisms in PPARD, PPARG and APM1 associated with four types of Traditional Chinese Medicine constitutions

Corresponding author: E-mail address: wangqi710@126.com (Qi Wang); E-mail address: cjxiao2009@gmail.com (Chunjie Xiao)

计量

出版历程

目录