Characterization of OP9 as authentic mesenchymal stem cell line

Jiao Gao^{a, b, #},
Xin-Long Yan^{a, b, #},
Ren Li^b,
Yi Liu^b,
Wenyan He^b,
Shengkun Sun^c,
Yu Zhang^d,
Bing Liu^b,
Jiaxiang Xiong^e
,,
Ning Mao^{a
, ,}

a.
Department of Cell Biology, Institute of Basic Medical Sciences, Beijing 100850, China
b.
Laboratory of Oncology, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China
c.
Department of Urology, PLA General Hospital, Beijing 100853, China
d.
Department of Immunology, Peking University Health Science Center, Beijing 100191, China
e.
Department of Physiology, Third Military Medical University, Chongqing 400038, China

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Corresponding author: E-mail address: maoning@nic.bmi.ac.cn (Ning Mao)

These authors contributed equally to this study.

摘要

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Abstract: Mesenchymal stem cells (MSCs) are multipotent stem cells capable of differentiating into various cell types, including osteocytes, chondrocytes, adipocytes, myocytes, and tenocytes. However, the difficulty or failure in expanding the mouse MSCs in vitro greatly hampered important research in animal models. The OP9, a stromal cell line from mouse bone marrow, has hematopoietic supportive capacity. Here, we report that the OP9 has the immunophenotype (CD45⁻, CD11b⁻, FLK-1⁻, CD31⁻, CD34⁻, CD44⁺, CD29⁺, Sca-1⁺, CD86⁻, and MHCII⁻) identical to canonical mouse MSCs. The expression of CD140a⁺, CD140b⁺, α-SMA⁺ and Calponin⁺ suggested the perivascular origin of OP9. Functionally, the OP9 had strong clonogenic ability and could be induced into osteocytes, chondrocytes and adipocytes. The lymphocyte transformation test (LTT) and mixed leukocyte reaction (MLR) showed that the OP9 could suppress T lymphocyte proliferation stimulated by nonspecific mitogens (PHA) or allogeneic lymphocytes (BALB/c T cells). Finally, the migration of OP9 could be efficiently induced by bFGF, IGF-1, IL-3, PDGF-BB, TGF-β1 and TGF-β3. In conclusion, the OP9 were bona fide MSCs, and such homogenous cell line will be helpful to delineate biological features of MSCs at the stem cell level.
- OP9 /
- mesenchymal stem cells /
- mouse /
- differentiation /
- immunoregulation
These authors contributed equally to this study.
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