Atp4b promoter directs the expression of Cre recombinase in gastric parietal cells of transgenic mice

Zengming Zhao^{a, #},
Ning Hou^{a, #},
Yanxun Sun^a,
Yan Teng^a,
Xiao Yang^{a, b
, ,}

a.
Genetic Laboratory of Development and Diseases, State Key Laboratory of Proteomics, Institute of Biotechnology, Beijing 100071, China
b.
Model Organism Division, E-Institutes of Shanghai Universities, Shanghai Jiao Tong University, Shanghai 200240, China

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Corresponding author: E-mail address: yangx@nic.bmi.ac.cn (Xiao Yang)

These authors contributed equally to this work.

摘要

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Abstract: Parietal cells are one of the largest epithelium cells of the mucous membrane of the stomach that secrete hydrochloric acid. To study the function of gastric parietal cells during gastric epithelium homeostasis, we generated a transgenic mouse line, namely, Atp4b-Cre, in which the expression of Cre recombinase was controlled by a 1.0 kb promoter of mouse (-subunit of H⁺-, K⁺-ATPase gene (Atp4b). In order to test the tissue distribution and excision activity of Cre recombinase in vivo, the Atp4b-Cre transgenic mice were bred with the reporter strainROSA26 and a mouse strain that carries Smad4 conditional alleles (). Multiple-tissue PCR of mice revealed that the recombination only happened in the stomach. As indicated by LacZ staining, ROSA26;Atp4b-Cre double transgenic mice showed efficient expression of Cre recombinase within the gastric parietal cells. These results showed that this Atp4b-Cre mouse line could be used as a powerful tool to achieve conditional gene knockout in gastric parietal cells.
- parietal cells /
- Cre recombinase /
- transgenic mouse
These authors contributed equally to this work.
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参考文献(21)

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