A nuclear-encoded mitochondrial gene AtCIB22 is essential for plant development in Arabidopsis

Lihua Han^a,
Genji Qin^b,
Dingming Kang^a,
Zhangliang Chen^{a, b},
Hongya Gu^{b, c},
Li-Jia Qu^{b, c
, ,}

a.
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China
b.
National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, China
c.
The National Plant Gene Research Center (Beijing), Beijing 100101, China

More Information

Corresponding author: E-mail address: qulj@pku.edu.cn (Li-Jia Qu)

摘要

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Abstract: Complex I (the NADH:ubiquinone oxidoreductase) of the mitochondrial respiratory chain is a complicated, multi-subunit, membrane-bound assembly and contains more than 40 different proteins in higher plants. In this paper, we characterize the Arabidopsis homologue (designated as AtCIB22) of the B22 subunit of eukaryotic mitochondrial Complex I. AtCIB22 is a single-copy gene and is highly conserved throughout eukaryotes. AtCIB22 protein is located in mitochondria and theAtCIB22 gene is widely expressed in different tissues. Mutant Arabidopsis plants with a disrupted AtCIB22 gene display pleiotropic phenotypes including shorter roots, smaller plants and delayed flowering. Stress analysis indicates that the AtCIB22 mutants' seed germination and early seedling growth are severely inhibited by sucrose deprivation stress but more tolerant to ethanol stress. Molecular analysis reveals that in moderate knockdown AtCIB22 mutants, genes including cell redox proteins and stress related proteins are significantly up-regulated, and that in severe knockdown AtCIB22 mutants, the alternative respiratory pathways including NDA1, NDB2, AOX1a and AtPUMP1 are remarkably elevated. These data demonstrate that AtCIB22 is essential for plant development and mitochondrial electron transport chains in Arabidopsis. Our findings also enhance our understanding about the physiological role of Complex I in plants.
- Arabidopsis /
- mitochondria /
- Complex I /
- B22 subunit /
- ethanol treatment /
- alternative oxidase /
- uncoupling protein

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