Additive and additive × additive interaction make important contributions to spikelets per panicle in rice near isogenic (Oryza sativa L.) lines

National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China

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Corresponding author: E-mail address: yzxing@mail.hzau.edu.cn (Yongzhong Xing)

These authors contributed equally to this work.

摘要

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Abstract: Epistasis plays an important role in the genetic basis of rice yield traits. Taking interactions into account in breeding programs will help the development of high-yielding rice varieties. In this study, three sets of near isogenic lines (NILs) targeting three QTLs for spikelets per panicle (SPP), namely qSPP1, qSPP2 and qSPP7, which share the same Zhenshan 97 genetic background, were used to produce an F₂ population in which the three QTLs segregated simultaneously. The genotypes of the individual F₂ plants at the three QTLs were replaced with three markers that are closely linked to the corresponding QTLs. These QTLs were validated in the F₂ and F₃ populations at the single marker level. qSPP7 exhibited major pleiotropic effects on SPP, plant height and heading date. Multifactor analysis of variance was performed for the F₂ population and its progeny. Additive (additive interaction between qSPP2 and qSPP7 had significant effects on SPP in both the F₂ population and its progeny. Both additive and additive (additive interactions could explain about 73% of the total SPP phenotypic variance. The SPP performance of 27 three-locus combinations was ranked and favorable combinations were recommended for rice breeding in different ecosystems.
- QTL validation /
- phenotypic variance /
- pleiotropic effect /
- favorable combination
These authors contributed equally to this work.
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参考文献(37)

[1]	Ando, T., Lin, S.Y., Shomura, A. et al. RFLP mapping of gene controlling seed number per panicle on chromosome 1 in rice Breed. Res., 3 (2001),p. 44
[2]	Ashikari, M., Sakakibara, H., Lin, S.Y. et al. Cytokinin oxidase regulates rice grain production Science, 309 (2005),pp. 741-745
[3]	Baker, R.J.
[4]	Bateson, W. The progress of genetics since the rediscovery of Mendel's paper Progressus Rei Botannicae, 1 (1907),pp. 368-418
[5]	Carlborg, O., Haley, C.S. Epistasis: too often neglected in complex trait studies? Nat. Rev. Genet., 5 (2004),pp. 618-625
[6]	Causse, M., Chaib, J., Lecomte, L. et al. Both additivity and epistasis control the genetic variation for fruit quality traits in tomato Theor. Appl. Genet., 115 (2007),pp. 429-442
[7]	Cheng, S.H., Zhuang, J.Y., Fan, Y.Y. et al. Progress in research and development on hybrid rice: a super-domesticate in china Ann. Bot., 100 (2007),pp. 959-966
[8]	Ishikawa, T., Fujimoto, H., Kabaki, N. et al. Dry matter production before heading and determination of number of spikelets of rice cultivar “Takanari” Jpn. J. Crop. Sci., 68 (1999),pp. 63-70
[9]	Kato, T. Quantitative trait loci controlling the number of spikelets and component traits in rice: Their main effects and interaction with years Breed. Sci., 54 (2004),pp. 125-132
[10]	Kobayashi, K., Imaki, T., Horie, T. Relationship between the size of the apical dome at the panicle initiation and the panicle components in rice Plant Prod. Sci., 4 (2001),pp. 81-87
[11]	Li, Z., Pinson, S.R., Park, W.D. et al. Genetics, 145 (1997),pp. 453-465
[12]	Liao, C.Y., Wu, P., Hu, B. et al. Theor. Appl. Genet., 103 (2001),pp. 104-111
[13]	Luo, L.J., Li, Z.K., Mei, H.W. et al. Overdominant epistatic loci are the primary genetic basis of inbreeding depression and heterosis in rice. II. Grain yield components Genetics, 158 (2001),pp. 1755-1771
[14]	Lou, X.Y., Chen, G.B., Yan, L. et al. A generalized combinatorial approach for detecting gene-by-gene and gene-by-environment interactions with application to nicotine dependence Am. J. Hum. Genet., 80 (2007),pp. 1125-1137
[15]	Maclean, J.L., Dawe, D.C., Hardy, B. et al.
[16]	Melchinger, A.E., Utz, H.F., Schon, C.C. Genetic expectations of quantitative trait loci main and interaction effects obtained with the triple testcross design and their relevance for the analysis of heterosis Genetics, 178 (2008),pp. 2265-2274
[17]	Monforte, A.J., Tanksley, S.D. Fine mapping of a quantitative trait locus (QTL) from Lycopersicon hirsutum chromosome 1 affecting fruit characteristics and agronomic traits: breaking linkage among QTLs affecting different traits and dissection of heterosis for yield Theor. Appl. Genet., 100 (2000),pp. 471-479
[18]	Nagata, K., Fukuta, Y., Shimizu, H. et al. Breed. Sci., 52 (2002),pp. 259-273
[19]	Tai, T.H., Tanksley, S.D. A rapid and inexpensive method for isolation of total DNA from dehydrated plant tissue Plant Mol. Biol. Rep., 8 (1990),pp. 297-303
[20]	Tian, F., Zhu, Z.F., Zhang, B.S. et al. Theor. Appl. Genet., 113 (2006),pp. 619-629
[21]	Wang, J.K. Inclusive composite interval mapping of quantitative trait genes Acta Agron. Sin., 35 (2009),pp. 239-245
[22]	Wright, S. Genic and organismic selection Evolution, 34 (1980),pp. 825-843
[23]	Wu, K.S., Tanksley, S.D. Abundance, polymorphism and genetic mapping of microsatellites in rice Mol. Gen. Genet., 241 (1993),pp. 225-235
[24]	Xing, Y.Z., Tang, W.J., Xue, W.Y. et al. Theor. Appl. Genet., 116 (2008),pp. 789-796
[25]	Xing, Y.Z., Tan, Y.F., Hua, J.P. et al. Characterization of the main effects, epistatic effects and their environmental interactions of QTLs on the genetic basis of yield traits in rice Theor. Appl. Genet., 105 (2002),pp. 248-257
[26]	Xue, W.Y., Xing, Y.Z., Weng, X.Y. et al. Nat. Genet., 40 (2008),pp. 761-767
[27]	Yagi, T., Nagata, K., Fukuta, Y. et al. Breed. Sci., 51 (2001),pp. 53-56
[28]	Yamamoto, T., Lin, H., Sasaki, T. et al. Genetics, 154 (2000),pp. 885-891
[29]	Yonezawa, K.
[30]	Yoshida, S., Parao, F.T.
[31]	Yu, S.B., Li, J.X., Xu, C.G. et al. Importance of epistasis as the genetic basis of heterosis in an elite rice hybrid Proc. Natl. Acad. Sci. USA, 94 (1997),pp. 9226-9231
[32]	Zeng, Z.B. Theoretical basis of separation of multiple linked gene effects on mapping quantitative trait loci Proc. Natl. Acad. Sci. USA, 90 (1993),pp. 10972-10976
[33]	Zhang, Y.S., Luo, L.J., Xu, C.G. et al. Theor. Appl. Genet., 113 (2006),pp. 361-368
[34]	Zhang, Y.S., Bai, X.F. A simple, rapid and high-resolution banding method in polyacrylamide gels Hereditas (Beijing), 30 (2008),pp. 251-254
[35]	Zhang, Y.S., Luo, L.J., Liu, T.M. et al. Four rice QTL controlling number of spikelets per panicle expressed the characteristics of single Mendelian gene in near isogenic backgrounds Theor. Appl. Genet., 118 (2009),pp. 1035-1044
[36]	Zhang, Z.H., Chen, M.M., Tang, J. et al. Acta Agron. Sin., 28 (2002),pp. 86-89
[37]	Zhu, D.F., Cheng, S.H., Zhang, Y.P. et al. Analysis of status and constraints of rice production in the world Sci. Agric. Sin., 43 (2010),pp. 474-479