Suppression of edr2-mediated powdery mildew resistance, cell death and ethylene-induced senescence by mutations in ALD1 in Arabidopsis

Haozhen Nie^{a, b},
Yingying Wu^{a, b},
Chunpeng Yao^{a, b},
Dingzhong Tang^{a
, ,}

a.
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
b.
Graduate University of Chinese Academy of Sciences, Beijing 100049, China

More Information

Corresponding author: E-mail address: dztang@genetics.ac.cn (Dingzhong Tang)

摘要

- /
- /
- /
- /
Abstract: EDR2 is a negative regulator of the defense response and cell death in Arabidopsis. Loss-of-function of EDR2 leads to enhanced resistance to powdery mildew. To identify new components in the EDR2 signal transduction pathway, mutations that suppress edr2 resistant phenotypes were screened. Three mutants, edts5-1, edts5-2 and edts5-3 (edr two suppressor 5), were identified. The EDTS5 gene was identified by map-based cloning and previously was shown to encode an aminotransferase (ALD1). Therefore we renamed these three alleles ald1-10, ald1-11 and ald1-12, respectively. Mutations in ALD1 suppressed all edr2-mediated phenotypes, including powdery mildew resistance, programmed cell death and ethylene-induced senescence. Accumulation of hydrogen peroxide in edr2 was also suppressed by ald1 mutation. The expression of defense-related genes was up-regulated in the edr2 mutant, and the up-regulation of those genes in edr2 was suppressed in the edr2/ald1 double mutant. The ald1 single mutant displayed delayed ethylene-induced senescence. In addition, ald1 mutation suppressed edr1-mediated powdery mildew resistance, but could not suppress the edr1/edr2 double-mutant phenotype. These data demonstrate that ALD1 plays important roles in edr2-mediated defense responses, and senescence and revealed a crosstalk between ethylene and salicylic acid signaling mediated by ALD1 and EDR2.
- ALD1 /
- Defense signaling /
- EDR2 /
- Powdery mildew /
- Salicylic acid

HTML全文

参考文献(50)

[1]	Adam, L., Somerville, S.C. Plant J., 9 (1996),pp. 341-356
[2]	Bednarek, P., Pislewska-Bednarek, M., Svatos, A. et al. A glucosinolate metabolism pathway in living plant cells mediates broad-spectrum antifungal defense Science, 323 (2009),pp. 101-106
[3]	Boutrot, F., Segonzac, C., Chang, K.N. et al. Proc. Nat. Acad. Sci. U.S.A., 107 (2010),pp. 14502-14507
[4]	Cao, H., Glazebrook, J., Clarke, J.D. et al. Cell, 88 (1997),pp. 57-63
[5]	Chen, H., Xue, L., Chintamanani, S. et al. Plant Cell, 21 (2009),pp. 2527-2540
[6]	Clay, N.K., Adio, A.M., Denoux, C. et al. Science, 323 (2009),pp. 95-101
[7]	Clough, S.J., Bent, A.F. Plant J., 16 (1998),pp. 735-743
[8]	Collins, N.C., Thordal-Christensen, H., Lipka, V. et al. SNARE-protein-mediated disease resistance at the plant cell wall Nature, 425 (2003),pp. 973-977
[9]	Consonni, C., Humphry, M.E., Hartmann, H.A. et al. Conserved requirement for a plant host cell protein in powdery mildew pathogenesis Nat. Genet., 38 (2006),pp. 716-720
[10]	Falk, A., Feys, B.J., Frost, L.N. et al. Proc. Nat. Acad. Sci. U.S.A., 96 (1999),pp. 3292-3297
[11]	Ferrari, S., Plotnikova, J.M., De Lorenzo, G. et al. Plant J., 35 (2003),pp. 193-205
[12]	Frye, C.A., Innes, R.W. Plant Cell, 10 (1998),pp. 947-956
[13]	Frye, C.A., Tang, D., Innes, R.W. Negative regulation of defense responses in plants by a conserved MAPKK kinase Proc. Nat. Acad. Sci. U.S.A., 98 (2001),pp. 373-378
[14]	Glazebrook, J. Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens Annu. Rev. Phytopathol., 43 (2005),pp. 205-227
[15]	Glazebrook, J., Zook, M., Mert, F. et al. Genetics, 146 (1997),pp. 381-392
[16]	Greenberg, J.T. Programmed cell death in plant-pathogen interactions Annu. Rev. Plant Physiol. Plant Mol. Biol., 48 (1997),pp. 525-545
[17]	Greenberg, J.T., Silverman, F.P., Liang, H. Genetics, 156 (2000),pp. 341-350
[18]	Jacobs, A.K., Lipka, V., Burton, R.A. et al. Plant Cell, 15 (2003),pp. 2503-2513
[19]	Kessler, S.A., Shimosato-Asano, H., Keinath, N.F. et al. Conserved molecular components for pollen tube reception and fungal invasion Science, 330 (2010),pp. 968-971
[20]	Koch, E., Slusarenko, A. Plant Cell, 2 (1990),pp. 437-445
[21]	Kwon, C., Neu, C., Pajonk, S. et al. Co-option of a default secretory pathway for plant immune responses Nature, 451 (2008),pp. 835-840
[22]	Liang, H., Yao, N., Song, J.T. et al. Ceramides modulate programmed cell death in plants Genes Dev., 17 (2003),pp. 2636-2641
[23]	Lipka, V., Dittgen, J., Bednarek, P. et al. Science, 310 (2005),pp. 1180-1183
[24]	Loake, G., Grant, M. Salicylic acid in plant defence–the players and protagonists Curr. Opin. Plant Biol., 10 (2007),pp. 466-472
[25]	Lu, H., Salimian, S., Gamelin, E. et al. Plant J., 58 (2009),pp. 401-412
[26]	Nishimura, M.T., Stein, M., Hou, B.H. et al. Loss of a callose synthase results in salicylic acid-dependent disease resistance Science, 301 (2003),pp. 969-972
[27]	Ryals, J., Weymann, K., Lawton, K. et al. Plant Cell, 9 (1997),pp. 425-439
[28]	Schulze-Lefert, P., Vogel, J. Closing the ranks to attack by powdery mildew Trends Plant Sci., 5 (2000),pp. 343-348
[29]	Song, J.T., Lu, H., Greenberg, J.T. Plant Cell, 16 (2004),pp. 353-366
[30]	Song, J.T., Lu, H., McDowell, J.M. et al. Plant J., 40 (2004),pp. 200-212
[31]	Stein, M., Dittgen, J., Sanchez-Rodriguez, C. et al. Plant Cell, 18 (2006),pp. 731-746
[32]	Stone, S.L., Williams, L.A., Farmer, L.M. et al. Plant Cell, 18 (2006),pp. 3415-3428
[33]	Tang, D., Innes, R.W. Plant J., 32 (2002),pp. 975-983
[34]	Tang, D., Christiansen, K.M., Innes, R.W. Plant Physiol., 138 (2005),pp. 1018-1026
[35]	Tang, D., Ade, J., Frye, C.A. et al. Plant J., 44 (2005),pp. 245-257
[36]	Tang, D., Ade, J., Frye, C.A. et al. Plant J., 47 (2006),pp. 75-84
[37]	van Engelen, F.A., Molthoff, J.W., Conner, A.J. et al. pBINPLUS: an improved plant transformation vector based on pBIN19 Transgenic Res., 4 (1995),pp. 288-290
[38]	Vogel, J., Somerville, S. Proc. Natl. Acad. Sci. U.S.A., 97 (2000),pp. 1897-1902
[39]	Vogel, J.P., Raab, T.K., Schiff, C. et al. Plant Cell, 14 (2002),pp. 2095-2106
[40]	Vogel, J.P., Raab, T.K., Somerville, C.R. et al. Plant J., 40 (2004),pp. 968-978
[41]	Vorwerk, S., Schiff, C., Santamaria, M. et al. BMC Plant Biol., 7 (2007),p. 35
[42]	Wang, W., Wen, Y., Berkey, R. et al. Plant Cell, 21 (2009),pp. 2898-2913
[43]	Wang, W., Yang, X., Tangchaiburana, S. et al. Plant Cell, 20 (2008),pp. 3163-3179
[44]	Wawrzynska, A., Christiansen, K.M., Lan, Y. et al. Powdery mildew resistance conferred by loss of the ENHANCED DISEASE RESISTANCE1 protein kinase is suppressed by a missense mutation in KEEP ON GOING, a regulator of abscisic acid signaling Plant Physiol., 148 (2008),pp. 1510-1522
[45]	Wildermuth, M.C., Dewdney, J., Wu, G. et al. Isochorismate synthase is required to synthesize salicylic acid for plant defence Nature, 414 (2001),pp. 562-565
[46]	Xiao, S., Brown, S., Patrick, E. et al. Plant Cell, 15 (2003),pp. 33-45
[47]	Xiao, S., Ellwood, S., Calis, O. et al. Science, 291 (2001),pp. 118-120
[48]	Yang, X., Wang, W., Coleman, M. et al. Plant J., 60 (2009),pp. 539-550
[49]	Yao, N., Eisfelder, B.J., Marvin, J. et al. Plant J., 40 (2004),pp. 596-610
[50]	Zhou, N., Tootle, T.L., Tsui, F. et al. Plant Cell, 10 (1998),pp. 1021-1030