Deletion of the topoisomerase III gene in the hyperthermophilic archaeon Sulfolobus islandicus results in slow growth and defects in cell cycle control

Xiyang Li^a,
Li Guo^a,
Ling Deng^b,
Deqin Feng^a,
Yi Ren^a,
Yindi Chu^a,
Qunxin She^{b
, ,},
Li Huang^{a
, ,}

a.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
b.
Archaea Centre, Department of Biology, Copenhagen University, Ole Maaløes Vej 5, DK-2200N Copenhagen, Denmark

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Corresponding author: E-mail address: qunxin@bio.ku.dk (Qunxin She); E-mail address: huangl@sun.im.ac.cn (Li Huang)

摘要

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Abstract: Topoisomerase III (topo III), a type IA topoisomerase, is widespread in hyperthermophilic archaea. In order to interrogate the in vivo role of archaeal topo III, we constructed and characterized a topo III gene deletion mutant of Sulfolobus islandicus. The mutant was viable but grew more slowly than the wild-type strain, especially in a nutrient-poor medium. Flow cytometry analysis revealed changes of the mutant in growth cycle characteristics including an increase in proportion of cells containing either more than two genome equivalents or less than one genome equivalent in exponentially-growing cultures. As shown by fluorescence microscopy, a fraction of mutant cells in the cultures were drastically enlarged, and at least some of the enlarged cells were apparently capable of resuming cell division. The mutant also shows a different transcriptional profile from that of the wild-type strain. Our results suggest that the enzyme may serve roles in chromosomal segregation and control of the level of supercoiling in the cell.
- Hyperthermophilic archaea /
- Topoisomerase III /
- Phenotype

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doi: 10.1016/j.jgg.2011.05.001

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Deletion of the topoisomerase III gene in the hyperthermophilic archaeon Sulfolobus islandicus results in slow growth and defects in cell cycle control

Corresponding author: E-mail address: qunxin@bio.ku.dk (Qunxin She); E-mail address: huangl@sun.im.ac.cn (Li Huang)

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doi: 10.1016/j.jgg.2011.05.001

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Deletion of the topoisomerase III gene in the hyperthermophilic archaeon Sulfolobus islandicus results in slow growth and defects in cell cycle control

Corresponding author: E-mail address: qunxin@bio.ku.dk (Qunxin She); E-mail address: huangl@sun.im.ac.cn (Li Huang)

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