A cotton mitogen-activated protein kinase (GhMPK6) is involved in ABA-induced CAT1 expression and H2O2 production

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China

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Corresponding author: E-mail address: wenliangxu@yahoo.com.cn (Wen-Liang Xu); E-mail address: xbli@mail.ccnu.edu.cn (Xue-Bao Li)

摘要

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Abstract: The mitogen-activated protein kinase (MAPK) cascade is one of the major and evolutionally conserved signaling pathways and plays a pivotal role in the regulation of stress and developmental signals in plants. Here, we identified one gene, GhMPK6, encoding an MAPK protein in cotton. GFP fluorescence assay demonstrated that GhMAPK6 is a cytoplasm localized protein. Quantitative RT-PCR analysis revealed that mRNA accumulation of GhMPK6 was significantly promoted by abscisic acid (ABA). Overexpression of GhMPK6 gene in the T-DNA insertion mutant atmkk1 (SALK_015914) conferred a wild-type phenotype to the transgenic plants in response to ABA. Under ABA treatment, cotyledon greening/expansion in GhMPK6 transgenic lines and wild type was significantly inhibited, whereas the atmkk1 mutant showed a relatively high cotyledon greening/expansion ratio. Furthermore, CAT1 expression and H₂O₂ levels in leaves of GhMPK6 transgenic lines and wild type were remarkably higher than those of atmkk1 mutant with ABA treatment. Collectively, our results suggested that GhMPK6 may play an important role in ABA-induced CAT1 expression and H₂O₂ production.
- Mitogen-activated protein kinase (MAPK) /
- Abscisic acid (ABA) /
- Hypersensitivity

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[1]	Asai, S., Ohta, K., Yoshioka, H. Plant Cell, 20 (2008),pp. 1390-1406
[2]	Asai, T., Tena, G., Plotnikova, J. et al. Nature, 415 (2002),pp. 977-983
[3]	Baker, C.J., Orlandi, E.W. Active oxygen in plant pathogenesis Annu. Rev. Phytopathol., 33 (1995),pp. 299-321
[4]	Bögre, L., Calderini, O., Binarova, P. et al. A MAP kinase is activated late in plant mitosis and becomes localized to the plane of cell division Plant Cell, 11 (1999),pp. 101-113
[5]	Dai, Y., Wang, H.Z., Li, B.H. et al. Plant Cell, 18 (2006),pp. 308-320
[6]	Guan, L.M., Zhao, J., Scandalios, J.G. Plant J., 22 (2000),pp. 87-95
[7]	Hu, X., Jiang, M., Zhang, A. et al. Planta, 223 (2005),pp. 57-68
[8]	Jammes, F., Song, C., Shin, D.J. et al. MAP kinases MPK9 and MPK12 are preferentially expressed in guard cells and positively regulate ROS-mediated ABA signaling Proc. Natl. Acad. Sci. USA, 106 (2009),pp. 20520-20525
[9]	Jiang, J., Wang, P.T., An, G.Y. et al. Plant Cell Rep., 27 (2008),pp. 377-385
[10]	Jonak, C., Ligterink, W., Hirt, H. MAP kinase in plant signal transduction Cell Mol. Life. Sci., 55 (1999),pp. 204-213
[11]	Levine, A., Tenhaken, R., Dixon, R. et al. Cell, 79 (1994),pp. 583-593
[12]	Low, P.S., Merida, J.R., Scandalios, J.G. The oxidative burst in plant defense Physiol. Plant, 96 (1996),pp. 533-542
[13]	Li, X.B., Cai, L., Cheng, N.H. et al. Plant Physiol., 130 (2002),pp. 666-674
[14]	Li, X.B., Fan, X.P., Wang, X.L. et al. Plant Cell, 17 (2005),pp. 859-875
[15]	Li, D.D., Wu, Y.J., Ruan, X.M. et al. Expressions of three cotton genes encoding the PIP proteins are regulated in root development and in response to stresses Plant Cell Rep., 28 (2009),pp. 291-300
[16]	Li, H.B., Qin, Y.M., Pang, Y. et al. A cotton ascorbate peroxidase is involved in hydrogen peroxide homeostasis during fibre cell development New Phytol., 175 (2007),pp. 462-471
[17]	Munnik, T., Ligterink, W., Meskiene, I. et al. Distinct osmo-sensing protein kinase pathways are involved in signalling moderate and severe hyper-osmotic stress Plant J., 20 (1999),pp. 381-388
[18]	Murphy, L.O., Smith, S., Chen, R.H. et al. Molecular interpretation of ERK signal duration by immediate early gene products Nat. Cell. Biol., 4 (2002),pp. 556-564
[19]	Nakagami, H., Pitzschke, A., Hirt, H. Emerging MAP kinase pathways in plant stress signaling Trends Plant Sci., 10 (2005),pp. 339-346
[20]	Neuenschwander, U., Vernooij, B., Fridrich, L. et al. Is hydrogen peroxide a second messenger of salicylic acid in systemic acquired resistance? Plant J., 8 (1995),pp. 227-233
[21]	Orozco-Cardenas, M., Ryan, C.A. Proc. Natl. Acad. Sci. USA, 96 (1999),pp. 6553-6557
[22]	Ortiz-Masia, D., Perez-Amador, M.A., Carbonell, P. et al. Planta, 227 (2008),pp. 1333-1342
[23]	Park, S.Y., Ryu, S.H., Jang, I.C. et al. Molecular cloning of a cytosolic ascorbate peroxidase cDNA from cell cultures of sweetpotato and its expression in response to stress Mol. Genet. Genomics, 271 (2004),pp. 339-346
[24]	Pitzschke, A., Hirt, H. Mitogen-activated protein kinases and reactive oxygen species signaling in plants Plant Physiol., 141 (2006),pp. 351-356
[25]	Ren, D., Yang, H., Zhang, S. J. Biol. Chem., 277 (2002),pp. 559-565
[26]	Reyna, N.S., Yang, Y.N. Mol. Plant Microbe Interact., 19 (2006),pp. 530-540
[27]	Schoenbeck, M.A., Samac, D.A., Fedorova, M. et al. Mol. Plant Microbe Interact., 12 (1999),pp. 882-893
[28]	Shi, J., An, H.L., Zhang, L. et al. Plant Mol. Biol., 74 (2010),pp. 1-17
[29]	Teige, M., Scheikl, E., Eulgem, T. et al. Mol. Cell, 15 (2004),pp. 141-152
[30]	Vanderauwera, S., Zimmermann, P., Rombauts, S. et al. Plant Physiol., 139 (2005),pp. 806-821
[31]	Wang, M.M., Zhang, Y., Wang, J. et al. J. Biochem. Mol. Biol., 40 (2007),pp. 325-332
[32]	Wang, P.C., Du, Y.Y., Li, Y. et al. Plant Cell, 22 (2010),pp. 2981-2998
[33]	Wang, P.T., Song, C.P. Guard-cell signalling for hydrogen peroxide and abscisic acid New Phytol., 178 (2008),pp. 703-718
[34]	Xing, Y., Jia, W., Zhang, J.H. Plant J., 54 (2008),pp. 440-451
[35]	Yoo, S.D., Cho, Y.H., Tena, G. et al. Nature, 451 (2008),pp. 789-795
[36]	Yoshioka, H., Numata, N., Nakajima, K. et al. Plant Cell Physiol., 42 (2003),pp. 1012-1016
[37]	Yuasa, T., Ichimura, K., Mizoguchi, T. et al. Plant Cell Physiol., 42 (2001),pp. 1012-1016
[38]	Zhang, A.Y., Jiang, M.Y., Zhang, J.H. et al. Nitric oxide induced by hydrogen peroxide mediates abscisic acid-induced activation of the mitogen-activated protein kinase cascade involved in antioxidant defense in maize leaves New Phytol., 175 (2007),pp. 36-50
[39]	Zhang, D.Y., Zhang, T.Z., Guo, W.Z. J. Plant Physiol., 167 (2010),pp. 393-399
[40]	Zhang, S., Klessig, D.F. MAPK cascades in plant defense signaling Trends Plant Sci., 6 (2001),pp. 520-527
[41]	Zhu, J.K. Salt and drought stress signal transduction in plants Annu. Rev. Plant Biol., 53 (2002),pp. 247-273
[42]	Zong, X.J., Li, D.P., Gu, L.K. et al. Planta, 229 (2009),pp. 485-495

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doi: 10.1016/j.jgg.2011.10.003

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A cotton mitogen-activated protein kinase (GhMPK6) is involved in ABA-induced CAT1 expression and H2O2 production

Corresponding author: E-mail address: wenliangxu@yahoo.com.cn (Wen-Liang Xu); E-mail address: xbli@mail.ccnu.edu.cn (Xue-Bao Li)

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doi: 10.1016/j.jgg.2011.10.003

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A cotton mitogen-activated protein kinase (GhMPK6) is involved in ABA-induced CAT1 expression and H2O2 production

Corresponding author: E-mail address: wenliangxu@yahoo.com.cn (Wen-Liang Xu); E-mail address: xbli@mail.ccnu.edu.cn (Xue-Bao Li)

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