A High-Throughput Method for Screening Arabidopsis Mutants with Disordered Abiotic Stress-Induced Calcium Signal

Zhen Pan^{a, b},
Yang Zhao^b,
Yuan Zheng^b,
Juntao Liu^b,
Xiangning Jiang^{a, c},
Yan Guo^{b
, ,}

a.
College of Life Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
b.
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China
c.
National Engineering Laboratory of Tree Breeding, The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration, Beijing 100083, China

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Corresponding author: E-mail address: guoyan@cau.edu.cn (Yan Guo)

摘要

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Abstract: It is established that different stresses cause signal-specific changes in cellular Ca²⁺ level, which function as messengers in modulating diverse physiological processes. These calcium signals are important for stress adaptation. Though numbers of downstream components of calcium signal cascades have been identified, upstream events in calcium signal remain elusive, specifically components required for calcium signal generation due to the lack of high-throughput genetic assay. Here, we report the development of an easy and efficient method in a forward genetic screen for Ca²⁺ signals-deficient mutants in Arabidopsis thaliana. Using this method, 121 mutants with disordered NaCl- and H₂O₂-induced Ca²⁺ signals are isolated.
- Mutant screen method /
- Calcium signal /
- Abiotic stress

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