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A Systematic Phenotypic Screen of F-box Genes Through a Tissue-specific RNAi-based Approach in Drosophila

Wen Dui,  Wei Lu,  Jun Ma,  Renjie Jiao

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文章导航 > Journal of Genetics and Genomics  > 2012 >  39(8): 397-413
Wen Dui, Wei Lu, Jun Ma, Renjie Jiao. A Systematic Phenotypic Screen of F-box Genes Through a Tissue-specific RNAi-based Approach in Drosophila[J]. Journal of Genetics and Genomics, 2012, 39(8): 397-413. doi: 10.1016/j.jgg.2012.05.009
Citation: Wen Dui, Wei Lu, Jun Ma, Renjie Jiao. A Systematic Phenotypic Screen of F-box Genes Through a Tissue-specific RNAi-based Approach in Drosophila[J]. Journal of Genetics and Genomics, 2012, 39(8): 397-413. doi: 10.1016/j.jgg.2012.05.009
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doi: 10.1016/j.jgg.2012.05.009
  • a, b, c, 
  • a, 
  • c, d, , , 
  • a, , 

A Systematic Phenotypic Screen of F-box Genes Through a Tissue-specific RNAi-based Approach in Drosophila

  • a.

    State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Datun Road 15, Beijing 100101, China

  • b.

    Graduate School of Chinese Academy of Sciences, Beijing 100080, China

  • c.

    Division of Biomedical Informatics, Cincinnati Children's Research Foundation, 3333 Burnet Avenue, Cincinnati, OH 45229, USA

  • d.

    Division of Developmental Biology, Cincinnati Children's Research Foundation, 3333 Burnet Avenue, Cincinnati, OH 45229, USA

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    Abstract: F-box proteins are components of the SCF (SkpA-Cullin 1-F-box) E3 ligase complexes, acting as the specificity-determinants in targeting substrate proteins for ubiquitination and degradation. In humans, at least 22 out of 75 F-box proteins have experimentally documented substrates, whereas in Drosophila 12 F-box proteins have been characterized with known substrates. To systematically investigate the genetic and molecular functions of F-box proteins in Drosophila, we performed a survey of the literature and databases. We identified 45 Drosophila genes that encode proteins containing at least one F-box domain. We collected publically available RNAi lines against these genes and used them in a tissue-specific RNAi-based phenotypic screen. Here, we present our systematic phenotypic dataset from the eye, the wing and the notum. This dataset is the first of its kind and represents a useful resource for future studies of the molecular and genetic functions of F-box genes in Drosophila. Our results show that, as expected, F-box genes in Drosophila have regulatory roles in a diverse array of processes including cell proliferation, cell growth, signal transduction, and cellular and animal survival.
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出版历程
  • 收稿日期:  2012-04-23
  • 录用日期:  2012-05-30
  • 修回日期:  2012-05-25
  • 网络出版日期:  2012-07-14
  • 刊出日期:  2012-08-20

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