The Non-Coding RNA Llme23 Drives the Malignant Property of Human Melanoma Cells

Chuan-Fang Wu^{a, #},
Guang-Hong Tan^{b, #},
Cheng-Chuan Ma^a,
Ling Li^{a
, ,}

a.
Center for Functional Genomics and Bioinformatics, Ministry of Education, Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, China
b.
Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical College, Haikou 571101, China

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Corresponding author: E-mail address: lingli1980@scu.edu.cn (Ling Li)

These authors contributed equally to this work.

摘要

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Abstract: Several lines of evidence support the notion that increased RNA-binding ability of polypyrimidine tract-binding (PTB) protein-associated splicing factor (PSF) and aberrant expression of long non-coding RNAs (lncRNAs) are associated with mouse and human tumors. To identify the PSF-binding lncRNA involved in human oncogenesis, we screened a nuclear RNA repertoire of human melanoma cell line, YUSAC, through RNA-SELEX affinity chromatography. A previously unreported lncRNA, termed as Llme23, was found to bind immobilized PSF resin. The specific binding of Llme23 to both recombinant and native PSF protein was confirmed in vitro and in vivo. The expression of PSF-binding Llme23 is exclusively detected in human melanoma lines. Knocking down Llme23 remarkably suppressed the malignant property of YUSAC cells, accompanied by the repressed expression of proto-oncogene Rab23. These results may indicate that Llme23 can function as an oncogenic RNA and directly associate the PSF-binding lncRNA with human melanoma.
- LncRNA /
- Llme23 /
- PSF /
- Melanoma /
- Rab23
These authors contributed equally to this work.
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