Arabidopsis PTD Is Required for Type I Crossover Formation and Affects Recombination Frequency in Two Different Chromosomal Regions

Pingli Lu^{a, b
, ,},
Asela J. Wijeratne^{b, #},
Zhengjia Wang^{b, c},
Gregory P. Copenhaver^{d, e},
Hong Ma^{a
, ,}

a.
Institute of Plant Biology, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China
b.
Department of Biology and the Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USA
c.
School of Forestry and Biotechnology, Zhejiang A&F University, Linan 311300, China
d.
Department of Biology and the Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
e.
Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599-3280, USA

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Corresponding author: E-mail address: pinglilu@fudan.edu.cn (Pingli Lu); E-mail address: hongma@fudan.edu.cn (Hong Ma)

Current address: Molecular and Cellular Imaging Center (MCIC), Ohio State University/OARDC, Wooster, OH 44691, USA.

摘要

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Abstract: In eukaryotes, crossovers together with sister chromatid cohesion maintain physical association between homologous chromosomes, ensuring accurate chromosome segregation during meiosis I and resulting in exchange of genetic information between homologues. The Arabidopsis PTD (Parting Dancers) gene affects the level of meiotic crossover formation, but its functional relationships with other core meiotic genes, such as AtSPO11-1, AtRAD51, and AtMSH4, are unclear; whether PTD has other functions in meiosis is also unknown. To further analyze PTD function and to test for epistatic relationships, we compared the meiotic chromosome behaviors of Atspo11-1 ptd and Atrad51 ptd double mutants with the relevant single mutants. The results suggest that PTD functions downstream of AtSPO11-1 and AtRAD51 in the meiotic recombination pathway. Furthermore, we found that meiotic defects in rck ptd and Atmsh4 ptd double mutants showed similar meiotic phenotypes to those of the relevant single mutants, providing genetic evidences for roles of PTD and RCK in the type I crossovers pathway. Moreover, we employed a pollen tetrad-based fluorescence method and found that the meiotic crossover frequencies in two genetic intervals were significantly reduced from 6.63% and 22.26% in wild-type to 1.14% and 6.36%, respectively, in the ptd-2 mutant. These results revealed new aspects ofPTD function in meiotic crossover formation.
- Meiosis /
- Crossover /
- Recombination frequency
Current address: Molecular and Cellular Imaging Center (MCIC), Ohio State University/OARDC, Wooster, OH 44691, USA.
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doi: 10.1016/j.jgg.2014.02.001

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Arabidopsis PTD Is Required for Type I Crossover Formation and Affects Recombination Frequency in Two Different Chromosomal Regions

Corresponding author: E-mail address: pinglilu@fudan.edu.cn (Pingli Lu); E-mail address: hongma@fudan.edu.cn (Hong Ma)

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doi: 10.1016/j.jgg.2014.02.001

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Arabidopsis PTD Is Required for Type I Crossover Formation and Affects Recombination Frequency in Two Different Chromosomal Regions

Corresponding author: E-mail address: pinglilu@fudan.edu.cn (Pingli Lu); E-mail address: hongma@fudan.edu.cn (Hong Ma)

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