Drosophila heparan sulfate 3-O sulfotransferase B Null Mutant Is Viable and Exhibits No Defects in Notch Signaling

Yueqin Guo^{a, b},
Ying Feng^{c, d},
Zhouhua Li^{a, e
, ,},
Xinhua Lin^{a, f
, ,}

a.
State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
b.
University of Chinese Academy of Sciences, Beijing 100049, China
c.
School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Wenzhou 325000, China
d.
State Key Laboratory of Stress Cell Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361005, China
e.
School of Life Sciences, Capital Normal University, Beijing 100048, China
f.
Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA

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Corresponding author: E-mail address: zhli@cnu.edu.cn (Zhouhua Li); E-mail address: xinhua.lin@ioz.ac.cn (Xinhua Lin)

摘要

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Abstract: Heparan sulfate proteoglycans (HSPGs) are critically involved in a variety of biological events. The functions of HSPGs are determined by the nature of the core proteins and modifications of heparan sulfate (HS) glycosaminoglycan (GAG) chains. The distinct O-sulfotransferases are important for nonrandom modifications at specific positions. Two HS 3-O sulfotransferase (Hs3st) genes, Hs3st-A and Hs3st-B, were identified in Drosophila. Previous experiments using RNA interference (RNAi) suggested that Hs3st-B was required for Notch signaling. Here, we generated a null mutant of Hs3st-B via ends-out gene targeting and examined its role(s) in development. We found that homozygous Hs3st-B mutants have no neurogenic defects or alterations in the expression of Notch signaling target gene. Thus, our results strongly argue against an essential role for Hs3st-B in Notch signaling. Moreover, we have generated two independent Hs3st-A RNAi lines which worked to deplete Hs3st-A. Importantly, Hs3st-A RNAi combined with Hs3st-B mutant flies did not alter the expression of Notch signaling components, arguing that both Hs3st-A and Hs3st-B were not essential for Notch signaling. The establishment of Hs3st-B mutant and effective Hs3st-A RNAi lines provides essential tools for further studies of the physiological roles of Hs3st-A and Hs3st-B in development and homeostasis.
- heparan sulfate proteoglycans /
- Notch

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doi: 10.1016/j.jgg.2014.04.006

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Drosophila heparan sulfate 3-O sulfotransferase B Null Mutant Is Viable and Exhibits No Defects in Notch Signaling

Corresponding author: E-mail address: zhli@cnu.edu.cn (Zhouhua Li); E-mail address: xinhua.lin@ioz.ac.cn (Xinhua Lin)

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doi: 10.1016/j.jgg.2014.04.006

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Drosophila heparan sulfate 3-O sulfotransferase B Null Mutant Is Viable and Exhibits No Defects in Notch Signaling

Corresponding author: E-mail address: zhli@cnu.edu.cn (Zhouhua Li); E-mail address: xinhua.lin@ioz.ac.cn (Xinhua Lin)

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