The Sterile 20-Like Kinase Tao Controls Tissue Homeostasis by Regulating the Hippo Pathway in Drosophila Adult Midgut

Xudong Huang^{a, #},
Lai Shi^{b, #},
Jun Cao^a,
Fangfei He^a,
Renling Li^a,
Yan Zhang^b,
Shuang Miao^c,
Longjin Jin^c,
Jia Qu^a,
Zhouhua Li^{b, d
, ,},
Xinhua Lin^{a, b, e
, ,}

a.
School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, Wenzhou 325000, China
b.
State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
c.
School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325000, China
d.
School of Life Sciences, Capital Normal University, Beijing 100048, China
e.
Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati 45229, USA

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Corresponding author: E-mail address: zhli@cnu.edu.cn (Zhouhua Li); E-mail address: xinhua.lin@ioz.ac.cn (Xinhua Lin)

These authors contributed equally to this work.

摘要

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Abstract: The proliferation and differentiation of adult stem cells must be tightly controlled in order to maintain resident tissue homeostasis. Dysfunction of stem cells is implicated in many human diseases, including cancer. However, the regulation of stem cell proliferation and differentiation is not fully understood. Here we show that the sterile-like 20 kinase, Tao, controls tissue homeostasis by regulating the Hippo pathway in theDrosophila adult midgut. Depletion of Tao in the progenitors leads to rapid intestinal stem cell (ISC) proliferation and midgut homeostasis loss. Meanwhile, we find that the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signaling activity and cytokine production are significantly increased, resulting in stimulated ISC proliferation. Furthermore, expression of the Hippo pathway downstream targets, Diap1 and bantam, is dramatically increased in Tao knockdown intestines. Consistently, we show that the Yorkie (Yki) acts downstream of Tao to regulate ISC proliferation. Together, our results provide insights into our understanding of the mechanisms of stem cell proliferation and tissue homeostasis control.
- Stem cell /
- Midgut /
- Tissue homeostasis /
- Tao /
- Hippo
These authors contributed equally to this work.
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doi: 10.1016/j.jgg.2014.05.007

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The Sterile 20-Like Kinase Tao Controls Tissue Homeostasis by Regulating the Hippo Pathway in Drosophila Adult Midgut

Corresponding author: E-mail address: zhli@cnu.edu.cn (Zhouhua Li); E-mail address: xinhua.lin@ioz.ac.cn (Xinhua Lin)

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doi: 10.1016/j.jgg.2014.05.007

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The Sterile 20-Like Kinase Tao Controls Tissue Homeostasis by Regulating the Hippo Pathway in Drosophila Adult Midgut

Corresponding author: E-mail address: zhli@cnu.edu.cn (Zhouhua Li); E-mail address: xinhua.lin@ioz.ac.cn (Xinhua Lin)

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