SPOROCYTELESS Is a Novel Embryophyte-Specific Transcription Repressor that Interacts with TPL and TCP Proteins in Arabidopsis

Guang-Hui Chen^{a, b},
Jia-Ying Sun^{a, b},
Man Liu^a,
Jie Liu^a,
Wei-Cai Yang^{a
, ,}

a.
State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
b.
The University of Chinese Academy of Sciences, 19 Yuquan Road, Shijingshan District, Beijing 100049, China

More Information

Corresponding author: E-mail address: wcyang@genetics.ac.cn (Wei-Cai Yang)

摘要

- /
- /
- /
Abstract: Germlines in plants are formed de novo during post-embryonic development, while little is known about the mechanism that controls this process. In Arabidopsis, the earliest gene controlling this process is SPOROCYTELESS (SPL). A decade ago, we showed that loss of SPL function abolished sporogenesis in both male and female organs of Arabidopsis. However, its function is unclear up to now. In this study, we showed that SPL belongs to a novel transcription repressor family specific in embryophyte, which consists of 173 members in the land plants so far. All of them contain a conserved SPL-motif in their N-terminal and an ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif in the C-terminal, therefore designated as SPL-like, EAR-containing proteins (SPEARs). Consistently, SPL acts as a transcriptional repressor in yeast and tobacco cells, and SPEAR proteins are able to form homodimer and/or heterodimer with each otherin vitro. Furthermore, SPEARs interact with the TOPLESS (TPL) co-repressors via the EAR motif and TCP family transcription factors in yeast cells. Together, we propose that SPL and SPEARs most likely belong to a novel transcription repressor family in land plants which may play a variety of developmental roles in plants.
- Germline specification /
- Sporogenesis /
- SPL /
- Transcription repressor

HTML全文

参考文献(39)

[1]	Bencivenga, S., Simonini, S., Benkova, E. et al. Plant Cell, 24 (2012),pp. 2886-2897
[2]	Berr, A., McCallum, E.J., Menard, R. et al. Plant Cell, 22 (2010),pp. 3232-3248
[3]	Causier, B., Ashworth, M., Guo, W.J. et al. Plant Physiol., 158 (2012),pp. 423-438
[4]	Chaubal, R., Anderson, J.R., Trimnell, M.R. et al. Planta, 216 (2003),pp. 778-788
[5]	Chen, P.Y., Wang, C.K., Soong, S.C. et al. Complete sequence of the binary vector pBI121 and its application in cloning T-DNA insertion from transgenic plants Mol. Breeding, 11 (2003),pp. 287-293
[6]	Clark, S.E., Running, M.P., Meyerowitz, E.M. Development, 119 (1993),pp. 397-418
[7]	Clark, S.E., Running, M.P., Meyerowitz, E.M. Clavata3 is a specific regulator of shoot and floral meristem development affecting the same processes as Clavata1 Development, 121 (1995),pp. 2057-2067
[8]	Curtis, M.D., Grossniklaus, U. Plant Physiol., 133 (2003),pp. 462-469
[9]	Edgar, R.C. MUSCLE: multiple sequence alignment with high accuracy and high throughput Nucleic Acids Res., 32 (2004),pp. 1792-1797
[10]	Extavour, C.G., Akam, M. Mechanisms of germ cell specification across the metazoans: epigenesis and preformation Development, 130 (2003),pp. 5869-5884
[11]	Goodstein, D.M., Shu, S.Q., Howson, R. et al. Phytozome: a comparative platform for green plant genomics Nucleic Acids Res., 40 (2012),pp. D1178-D1186
[12]	Gu, X.F., Jiang, D.H., Yang, W.N. et al. PLoS Genet., 7 (2011)
[13]	Hord, C.H., Ma, H.
[14]	Hord, C.L.H., Chen, C.B., DeYoung, B.J. et al. Plant Cell, 18 (2006),pp. 1667-1680
[15]	Ito, T., Wellmer, F., Yu, H. et al. Nature, 430 (2004),pp. 356-360
[16]	Kagale, S., Rozwadowski, K. EAR motif-mediated transcriptional repression in plants an underlying mechanism for epigenetic regulation of gene expression Epigenetics, 6 (2011),pp. 141-146
[17]	Kelliher, T., Walbot, V. Hypoxia triggers meiotic fate acquisition in maize Science, 337 (2012),pp. 345-348
[18]	Kim, J., Harter, K., Theologis, A. Protein-protein interactions among the Aux/IAA proteins Proc. Natl. Acad. Sci. USA, 94 (1997),pp. 11786-11791
[19]	Krichevsky, A., Zaltsman, A., Lacroix, B. et al. Involvement of KDM1C histone demethylase-OTLD1 otubain-like histone deubiquitinase complexes in plant gene repression Proc. Natl. Acad. Sci. USA, 108 (2011),pp. 11157-11162
[20]	Kwak, S.H., Schiefelbein, J. Dev. Biol., 302 (2007),pp. 118-131
[21]	Li, L.C., Qin, G.J., Tsuge, T. et al. New Phytol., 179 (2008),pp. 751-764
[22]	Liscum, E., Reed, J.W. Genetics of Aux/IAA and ARF action in plant growth and development Plant Mol. Biol., 49 (2002),pp. 387-400
[23]	Liu, L.J., Zhang, Y.Y., Tang, S.Y. et al. Plant J., 61 (2010),pp. 893-903
[24]	Liu, X., Luo, M., Wu, K. Epigenetic interplay of histone modifications and DNA methylation mediated by HDA6 Plant Signal. Behav., 7 (2012),pp. 633-635
[25]	Liu, X.C., Yu, C.W., Duan, J. et al. Plant Physiol., 158 (2012),pp. 119-129
[26]	Nakagawa, T., Kurose, T., Hino, T. et al. Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation J. Biosci. Bioeng., 104 (2007),pp. 34-41
[27]	Pauwels, L., Barbero, G.F., Geerinck, J. et al. NINJA connects the co-repressor TOPLESS to jasmonate signalling Nature, 464 (2010),pp. 788-791
[28]	Sarojam, R., Sappl, P.G., Goldshmidt, A. et al. Plant Cell, 22 (2010),pp. 2113-2130
[29]	Schiefthaler, U., Balasubramanian, S., Sieber, P. et al. Proc. Natl. Acad. Sci. USA, 96 (1999),pp. 11664-11669
[30]	Scott, R.J., Spielman, M., Dickinson, H.G. Stamen structure and function Plant Cell, 16 (2004),pp. S46-S60
[31]	Sieber, P., Petrascheck, M., Barberis, A. et al. Plant Physiol., 135 (2004),pp. 2172-2185
[32]	Szemenyei, H., Hannon, M., Long, J.A. Science, 319 (2008),pp. 1384-1386
[33]	Tamura, K., Peterson, D., Peterson, N. et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods Mol. Biol. Evol., 28 (2011),pp. 2731-2739
[34]	Tao, Q., Guo, D.S., Wei, B.Y. et al. Plant Cell, 25 (2013),pp. 421-437
[35]	To, T.K., Kim, J.M., Matsui, A. et al. PLoS Genet., 7 (2011)
[36]	Wilson, Z.A., Zhang, D.B. J. Exp. Bot., 60 (2009),pp. 1479-1492
[37]	Xing, S., Zachgo, S. Plant J., 53 (2008),pp. 790-801
[38]	Yang, W.C., Ye, D., Xu, J. et al. Gene Dev., 13 (1999),pp. 2108-2117
[39]	Yu, C.W., Liu, X.C., Luo, M. et al. Plant Physiol., 156 (2011),pp. 173-184