A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila

Jiang Xu^{a, b, c, d},
Xingjie Ren^a,
Jin Sun^a,
Xia Wang^a,
Huan-Huan Qiao^a,
Bo-Wen Xu^a,
Lu-Ping Liu^{a, b},
Jian-Quan Ni^{a
, ,}

a.
Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China
b.
Tsinghua Fly Center, Tsinghua University, Beijing 100084, China
c.
School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China
d.
College of Bioengineering, Hubei University of Technology, Wuhan 430068, China

More Information

Corresponding author: E-mail address: nijq@mail.tsinghua.edu.cn (Jian-Quan Ni)

摘要

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Abstract: The last couple of years have witnessed an explosion in development of CRISPR-based genome editing technologies in cell lines as well as in model organisms. In this review, we focus on the applications of this popular system in Drosophila. We discuss the effectiveness of the CRISPR/Cas9 systems in terms of delivery, mutagenesis detection, parameters affecting efficiency, and off-target issues, with an emphasis on how to apply this powerful tool to characterize gene functions.
- CRISPR /
- Genome editing /
- Cas9 /
- sgRNA /
- Off-target

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参考文献(88)

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