Targeted Mutagenesis of Duplicated Genes in Caenorhabditis elegans Using CRISPR-Cas9

Suhong Xu^{a, #
, ,},
Zhiping Wang^{b, #},
Kyung Won Kim^b,
Yishi Jin^{b, c},
Andrew D. Chisholm^{a, b
, ,}

a.
Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
b.
Division of Biological Sciences, Section of Neurobiology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA
c.
Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093, USA

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Corresponding author: E-mail address: shxu@zju.edu.cn (Suhong Xu); E-mail address: chisholm@ucsd.edu (Andrew D. Chisholm)

Present address: School of Medicine, Zhejiang University, Hangzhou, China.

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Present address: School of Medicine, Zhejiang University, Hangzhou, China.

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[3]	Cong, L., Ran, F.A., Cox, D. et al. Multiplex genome engineering using CRISPR/Cas systems Science, 339 (2013),pp. 819-823
[4]	Farboud, B., Meyer, B.J. Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design Genetics, 199 (2015),pp. 959-971
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[10]	Schwarz, T.L. Mitochondrial trafficking in neurons Cold Spring Harb. Perspect. Biol. (2013),p. 5
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[12]	Singh, P., Schimenti, J.C., Bolcun-Filas, E. A mouse geneticist's practical guide to CRISPR applications Genetics, 199 (2015),pp. 1-15
[13]	Taylor, J.S., Raes, J. Duplication and divergence: the evolution of new genes and old ideas Annu. Rev. Genet., 38 (2004),pp. 615-643
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[17]	Xu, S., Chisholm, A.D. Dev. Cell, 31 (2014),pp. 48-60

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doi: 10.1016/j.jgg.2015.11.004

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Targeted Mutagenesis of Duplicated Genes in Caenorhabditis elegans Using CRISPR-Cas9

Corresponding author: E-mail address: shxu@zju.edu.cn (Suhong Xu); E-mail address: chisholm@ucsd.edu (Andrew D. Chisholm)

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doi: 10.1016/j.jgg.2015.11.004

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Targeted Mutagenesis of Duplicated Genes in Caenorhabditis elegans Using CRISPR-Cas9

Corresponding author: E-mail address: shxu@zju.edu.cn (Suhong Xu); E-mail address: chisholm@ucsd.edu (Andrew D. Chisholm)

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