Genetic Components of Heterosis for Seedling Traits in an Elite Rice Hybrid Analyzed Using an Immortalized F2 Population

National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China

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Corresponding author: E-mail address: qifazh@mail.hzau.edu.cn (Qifa Zhang)

摘要

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Abstract: Utilization of heterosis has greatly contributed to rice productivity in China and many Asian countries. Superior hybrids usually show heterosis at two stages: canopy development at vegetative stage and panicle development at reproductive stage resulting in heterosis in yield. Although the genetic basis of heterosis in rice has been extensively investigated, all the previous studies focused on yield traits at maturity stage. In this study, we analyzed the genetic basis of heterosis at seedling stage making use of an “immortalized F₂” population composed of 105 hybrids produced by intercrossing recombinant inbred lines (RILs) from a cross between Zhenshan 97 and Minghui 63, the parents of Shanyou 63, which is an elite hybrid widely grown in China. Eight seedling traits, seedling height, tiller number, leaf number, root number, maximum root length, root dry weight, shoot dry weight and total dry weight, were investigated using hydroponic culture. We analyzed single-locus and digenic genetic effects at the whole genome level using an ultrahigh-density SNP bin map obtained by population re-sequencing. The analysis revealed large numbers of heterotic effects for seedling traits including dominance, overdominance and digenic dominance (epistasis) in both positive and negative directions. Overdominance effects were prevalent for all the traits, and digenic dominance effects also accounted for a large portion of the genetic effects. The results suggested that cumulative small advantages of the single-locus effects and two-locus interactions, most of which could not be detected statistically, could explain the genetic basis of seedling heterosis of the F₁ hybrid.
- Heterosis /
- Seedling trait /
- Overdominance /
- Digenic dominance

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参考文献(35)

[1]	Barth, S., Busimi, A.K., Friedrich Utz, H. et al. Heynh. Heredity, 91 (2003),pp. 36-42
[2]	Cockerham, C.C. An extension of the concept of partitioning hereditary variance for analysis of covariances among relatives when epistasis is present Genetics, 39 (1954),pp. 859-882
[3]	Cui, H., Peng, B., Xing, Z. et al. Molecular dissection of seedling-vigor and associated physiological traits in rice Theor. Appl. Genet., 105 (2002),pp. 745-753
[4]	Cui, K., Huang, J., Xing, Y. et al. Physiol. Plant, 132 (2008),pp. 53-68
[5]	Frascaroli, E., Cane, M.A., Landi, P. et al. Classical genetic and quantitative trait loci analyses of heterosis in a maize hybrid between two elite inbred lines Genetics, 176 (2007),pp. 625-644
[6]	Hoecker, N., Keller, B., Piepho, H.P. et al. Theor. Appl. Genet., 112 (2006),pp. 421-429
[7]	Hua, J., Xing, Y., Wu, W. et al. Single-locus heterotic effects and dominance by dominance interactions can adequately explain the genetic basis of heterosis in an elite rice hybrid Proc. Natl. Acad. Sci. USA, 100 (2003),pp. 2574-2579
[8]	Hua, J.P., Xing, Y.Z., Xu, C.G. et al. Genetic dissection of an elite rice hybrid revealed that heterozygotes are not always advantageous for performance Genetics, 162 (2002),pp. 1885-1895
[9]	Huang, Y., Zhang, L., Zhang, J. et al. Heterosis and polymorphisms of gene expression in an elite rice hybrid as revealed by a microarray analysis of 9198 unique ESTs Plant Mol. Biol., 62 (2006),pp. 579-591
[10]	Kearsey, M.J., Pooni, H.S., Syed, N.H. Heredity, 91 (2003),pp. 456-464
[11]	Kusterer, B., Muminovic, J., Utz, H.F. et al. Genetics, 175 (2007),pp. 2009-2017
[12]	Kusterer, B., Piepho, H.P., Utz, H.F. et al. Genetics, 177 (2007),pp. 1839-1850
[13]	Li, L.Z., Lu, K.Y., Chen, Z.M. et al. Dominance, overdominance and epistasis condition the heterosis in two heterotic rice hybrids Genetics, 180 (2008),pp. 1725-1742
[14]	Li, Z.K., Luo, L.J., Mei, H.W. et al. Overdominant epistatic loci are the primary genetic basis of inbreeding depression and heterosis in rice. I. Biomass and grain yield Genetics, 158 (2001),pp. 1737-1753
[15]	Luo, L.J., Li, Z.K., Mei, H.W. et al. Overdominant epistatic loci are the primary genetic basis of inbreeding depression and heterosis in rice. II. Grain yield components Genetics, 158 (2001),pp. 1755-1771
[16]	Luo, X.J., Xin, X.Y., Yang, J.S. Genet. Res., 94 (2012),pp. 57-61
[17]	Ma, Q., Hedden, P., Zhang, Q. Heterosis in rice seedlings: its relationship to gibberellin content and expression of gibberellin metabolism and signaling genes Plant Physiol., 156 (2011),pp. 1905-1920
[18]	Melchinger, A.E., Piepho, H.P., Utz, H.F. et al. Genetics, 177 (2007),pp. 1827-1837
[19]	Meyer, R.C., Kusterer, B., Lisec, J. et al. Theor. Appl. Genet., 120 (2010),pp. 227-237
[20]	Meyer, R.C., Torjek, O., Becher, M. et al. Plant Physiol., 134 (2004),pp. 1813-1823
[21]	Semel, Y., Nissenbaum, J., Menda, N. et al. Overdominant quantitative trait loci for yield and fitness in tomato Proc. Natl. Acad. Sci. USA, 103 (2006),pp. 12981-12986
[22]	Snedecor, G.W., Cochran, W.G.
[23]	Syed, N.H., Chen, Z.J. Heredity, 94 (2005),pp. 295-304
[24]	Tollenaar, M., Ahmadzadeh, A., Lee, E.A. Physiological basis of heterosis for grain yield in maize Crop Sci., 44 (2004),pp. 2086-2094
[25]	Xiao, J., Li, J., Yuan, L. et al. Dominance is the major genetic basis of heterosis in rice as revealed by QTL analysis using molecular markers Genetics, 140 (1995),pp. 745-754
[26]	Xie, W., Feng, Q., Yu, H. et al. Parent-independent genotyping for constructing an ultrahigh-density linkage map based on population sequencing Proc. Natl. Acad. Sci. USA, 107 (2010),pp. 10578-10583
[27]	Xin, X.Y., Wang, W.X., Yang, J.S. et al. Breed. Sci., 61 (2011),pp. 380-388
[28]	Xing, Y.Z., Tan, Y.F., Hua, J.P. et al. Characterization of the main effects, epistatic effects and their environmental interactions of QTLs on the genetic basis of yield traits in rice Theor. Appl. Genet., 105 (2002),pp. 248-257
[29]	Xu, C.G., Li, X.Q., Xue, Y. et al. Comparison of quantitative trait loci controlling seedling characteristics at two seedling stages using rice recombinant inbred lines Theor. Appl. Genet., 109 (2004),pp. 640-647
[30]	Yoshida, S., Forno, D.A., Cock, J.
[31]	Yu, H., Xie, W., Wang, J. et al. Gains in QTL detection using an ultra-high density SNP map based on population sequencing relative to traditional RFLP/SSR markers PLoS One, 6 (2011),p. e17595
[32]	Yu, S.B., Li, J.X., Xu, C.G. et al. Importance of epistasis as the genetic basis of heterosis in an elite rice hybrid Proc. Natl. Acad. Sci. USA, 94 (1997),pp. 9226-9231
[33]	Zhai, R., Feng, Y., Wang, H. et al. Transcriptome analysis of rice root heterosis by RNA-Seq BMC Genomics, 14 (2013),p. 19
[34]	Zhang, H.Y., He, H., Chen, L.B. et al. A genome-wide transcription analysis reveals a close correlation of promoter INDEL polymorphism and heterotic gene expression in rice hybrids Mol. Plant, 1 (2008),pp. 720-731
[35]	Zhou, G., Chen, Y., Yao, W. et al. Genetic composition of yield heterosis in an elite rice hybrid Proc. Natl. Acad. Sci. USA, 109 (2012),pp. 15847-15852