Construction and Validation of a Dual-Transgene Vector System for Stable Transformation in Plants

Zhimin He^{a, b},
Bin Liu^c,
Xu Wang^b,
Mingdi Bian^d,
Reqing He^a,
Jindong Yan^a,
Ming Zhong^a,
Xiaoying Zhao^{a
, ,},
Xuanming Liu^{a, e
, ,}

a.
Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha 410082, China
b.
Department of Molecular, Cell & Developmental Biology, University of California, Los Angeles, CA 90095, USA
c.
Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
d.
College of Plant Sciences, Jilin University, Changchun 130062, China
e.
State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, China

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Corresponding author: E-mail address: xiaoyzhao@hnu.edu.cn (Xiaoying Zhao); E-mail address: xmL05@hnu.edu.cn (Xuanming Liu)

摘要

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Abstract: In this study, we constructed dual-transgene vectors (pDT1, pDT7, and pDT7G) that simultaneously co-expressed two genes in plants. ACTIN2 and UBQ10 promoters were used to control the expression of these two genes. The 4×Myc, 3×HA, and 3×Flag reporter genes allowed for the convenient identification of a tunable co-expression system in plants, whereas the dexamethasone (Dex) inducible reporter gene C-terminus of the glucocorticoid receptor (cGR) provided Dex-dependent translocation of the fusion gene between the nucleus and cytoplasm. The function of pDT vectors was validated using four pairwise genes in Nicotiana benthamiana or Arabidopsis thaliana. The co-expression efficiency of two genes from the pDT1 and pDT7G vectors was 35% and 42%, respectively, which ensured the generation of sufficient transgenic materials. These pDT vectors are simple, reliable, efficient, and time-saving tools for the co-expression of two genes through a single transformation event and can be used in the study of protein–protein interactions or multi-component complexes.
- Co-expression /
- Dual-transgene vector /
- pDT1 /
- pDT7 /
- pDT7G

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doi: 10.1016/j.jgg.2016.02.005

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Construction and Validation of a Dual-Transgene Vector System for Stable Transformation in Plants

Corresponding author: E-mail address: xiaoyzhao@hnu.edu.cn (Xiaoying Zhao); E-mail address: xmL05@hnu.edu.cn (Xuanming Liu)

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doi: 10.1016/j.jgg.2016.02.005

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Construction and Validation of a Dual-Transgene Vector System for Stable Transformation in Plants

Corresponding author: E-mail address: xiaoyzhao@hnu.edu.cn (Xiaoying Zhao); E-mail address: xmL05@hnu.edu.cn (Xuanming Liu)

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