Generation of thermosensitive male-sterile maize by targeted knockout of the ZmTMS5 gene

Jun Li^{1, 2, #},
Huawei Zhang^{3, #},
Xiaomin Si^{4, 5},
Youhui Tian^{6, 7},
Kunling Chen⁸,
Jinxing Liu⁹,
Huabang Chen¹⁰,
Caixia Gao^{11, 12
, ,}

1.
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
2.
University of Chinese Academy of Sciences, Beijing 100049, China
3.
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
4.
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
5.
University of Chinese Academy of Sciences, Beijing 100049, China
6.
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
7.
University of Chinese Academy of Sciences, Beijing 100049, China
8.
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
9.
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
10.
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
11.
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
12.
University of Chinese Academy of Sciences, Beijing 100049, China

More Information

Corresponding author: E-mail address: cxgao@genetics.ac.cn (Caixia Gao)

These authors contributed equally to this work.

摘要

These authors contributed equally to this work.

注释:

HTML全文

参考文献(19)

[1]	Bae, S., Park, J., Kim, J. Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases Bioinformatics, 30 (2014),pp. 1473-1475
[2]	Baldaufa, J.A., Marcona, C., Pascholda, A. et al. Nonsyntenic genes drive tissue-specific dynamics of differential, nonadditive and allelic expression patterns in maize hybrids Plant Physiol., 171 (2016),pp. 1144-1155
[3]	Boch, J., Scholze, H., Schornack, S. et al. Breaking the code of DNA binding specificity of TAL-type III effectors Science, 326 (2009),pp. 1509-1512
[4]	Bortesi, L., Fischer, R. The CRISPR/Cas9 system for plant genome editing and beyond Biotechnol. Adv., 33 (2015),pp. 41-52
[5]	Cong, L., Ran, F.A., Cox, D. et al. Multiplex genome engineering using CRISPR/Cas systems Science, 339 (2013),pp. 819-823
[6]	Duvick, D.N. Biotechnology in the 1930s: the development of hybrid maize Nat. Rev. Genet., 2 (2001),pp. 69-74
[7]	Frame, B.R., Zhang, H.Y., Cocciolone, S.M. et al. Production of transgenic maize from bombarded Type II callus: effect of gold particle size and callus morphology on transformation efficiency In Vitro Cell. Dev. Biol. Plant, 36 (2000),pp. 21-29
[8]	Gorbunova, V., Levy, A.A. Non-homologous DNA end joining in plant cells is associated with deletions and filler DNA insertions Nucleic Acids Res., 25 (1997),pp. 4650-4657
[9]	Jinek, M., Chylinski, K., Fonfara, I. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity Science, 337 (2012),pp. 816-821
[10]	Levings, C.S. III. Thoughts on cytoplasmic male sterility in CMS-T maize Plant Cell, 5 (1993),pp. 1285-1290
[11]	Li, Z., Liu, Z.B., Xing, A. et al. Cas9-guide RNA directed genome editing in soybean Plant Physiol., 169 (2015),pp. 960-970
[12]	Liang, Z., Zhang, K., Chen, K. et al. J. Genet. Genomics, 41 (2014),pp. 63-68
[13]	Mali, P., Yang, L., Esvelt, K.M. et al. RNA-guided human genome engineering via Cas9 Science, 339 (2013),pp. 823-826
[14]	Puchta, H., Dujon, B., Hohn, B. Two different but related mechanisms are used in plants for the repair of genomic double-strand breaks by homologous recombination Proc. Natl. Acad. Sci. U. S. A., 93 (1996),pp. 5055-5060
[15]	Shan, Q.W., Wang, Y.P., Li, J. et al. Targeted genome modification of crop plants using a CRISPR-Cas system Nat. Biotechnol., 31 (2013),pp. 686-688
[16]	Symington, L.S., Gautier, J. Double-strand break end resection and repair pathway choice Annu. Rev. Genet., 45 (2011),pp. 247-271
[17]	Wang, Y., Cheng, X., Shan, Q. et al. Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew Nat. Biotechnol., 32 (2014),pp. 947-951
[18]	Weeks, D.P., Spalding, M.H., Yang, B. Use of designer nucleases for targeted gene and genome editing in plants Plant Biotechnol. J., 14 (2016),pp. 483-495
[19]	Zhou, H., Zhou, M., Yang, Y.Z. et al. Nat. Commun., 5 (2014),p. 4884