Efficient generation of the mouse model with a defined point mutation through haploid cell-mediated gene editing

Leixin Wei^{1, 2, #},
Xiukun Wang^{3, #},
Suming Yang³,
Wen Yuan^{4
, ,},
Jinsong Li^{5
, ,}

1.
Department of Orthopaedic Surgery, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, China
2.
State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences; University of Chinese Academy of Sciences, Shanghai 200031, China
3.
State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences; University of Chinese Academy of Sciences, Shanghai 200031, China
4.
Department of Orthopaedic Surgery, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, China
5.
State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences; University of Chinese Academy of Sciences, Shanghai 200031, China

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Corresponding author: E-mail address: yuanwenspine@163.com (Wen Yuan); E-mail address: jsli@sibcb.ac.cn (Jinsong Li)

These authors contributed equally to this work.

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These authors contributed equally to this work.

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[1]	Inui, M., Miyado, M., Igarashi, M. et al. Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system Sci. Rep., 4 (2014),p. 5396
[2]	Ishii, A., Kurosawa, A., Saito, S. et al. Analysis of the role of homology arms in gene-targeting vectors in human cells PLoS One, 9 (2014),p. e108236
[3]	Mei, Y., Wang, Y., Chen, H. et al. Recent progress in CRISPR/Cas9 technology J. Genet. Genomics, 43 (2016),pp. 63-75
[4]	Niu, Y., Shen, B., Cui, Y. et al. Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos Cell, 156 (2014),pp. 836-843
[5]	Wang, H., Yang, H., Shivalila, C.S. et al. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering Cell, 153 (2013),pp. 910-918
[6]	Wang, K., Tang, X., Liu, Y. et al. Efficient generation of orthologous point mutations in pigs via CRISPR-assisted ssODN-mediated homology-directed repair Mol. Ther. Nucleic Acids, 5 (2016),p. e396
[7]	Wu, Y., Liang, D., Wang, Y. et al. Correction of a genetic disease in mouse via use of CRISPR-Cas9 Cell Stem Cell, 13 (2013),pp. 659-662
[8]	Xiao, J., Xu, M., Li, J. et al. Biochem. Biophys. Res. Commun., 291 (2002),pp. 101-110
[9]	Yang, H., Shi, L., Wang, B.A. et al. Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells Cell, 149 (2012),pp. 605-617
[10]	Zhong, C., Yin, Q., Xie, Z. et al. CRISPR-Cas9-mediated genetic screening in mice with haploid embryonic stem cells carrying a guide RNA library Cell Stem Cell, 17 (2015),pp. 221-232

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doi: 10.1016/j.jgg.2017.07.004

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Efficient generation of the mouse model with a defined point mutation through haploid cell-mediated gene editing

Corresponding author: E-mail address: yuanwenspine@163.com (Wen Yuan); E-mail address: jsli@sibcb.ac.cn (Jinsong Li)

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doi: 10.1016/j.jgg.2017.07.004

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Efficient generation of the mouse model with a defined point mutation through haploid cell-mediated gene editing

Corresponding author: E-mail address: yuanwenspine@163.com (Wen Yuan); E-mail address: jsli@sibcb.ac.cn (Jinsong Li)

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