Efficient generation of pink-fruited tomatoes using CRISPR/Cas9 system

Lei Deng^{1, #},
Hang Wang^{2, #},
Chuanlong Sun³,
Qian Li³,
Hongling Jiang³,
Minmin Du⁴,
Chang-Bao Li^{4
, ,},
Chuanyou Li^{5, 6
, ,}

1.
State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
2.
State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, China
3.
State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
4.
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
5.
State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
6.
State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, China

More Information

Corresponding author: E-mail address: lichangbao@nercv.org (Chang-Bao Li); E-mail address: cyli@genetics.ac.cn (Chuanyou Li)

These authors contributed equally to this work.

摘要

These authors contributed equally to this work.

注释:

HTML全文

参考文献(20)

[1]	Adato, A., Mandel, T., Mintz-Oron, S. et al. PLoS Genet., 5 (2009),p. e1000777
[2]	Ballester, A.R., Molthoff, J., de Vos, R. et al. Biochemical and molecular analysis of pink tomatoes: deregulated expression of the gene encoding transcription factor S1MYB12 leads to pink tomato fruit color Plant Physiol., 152 (2010),pp. 71-84
[3]	Brooks, C., Nekrasov, V., Lippman, Z.B. et al. Efficient gene editing in tomato in the first generation using the clustered regularly interspaced short palindromic repeats/CRISPR-associated9 system Plant Physiol., 166 (2014),pp. 1292-1297
[4]	Cong, L., Ran, F.A., Cox, D. et al. Multiplex genome engineering using CRISPR/Cas systems Science, 339 (2013),pp. 819-823
[5]	Du, M.M., Zhao, J.H., Tzeng, D.T.W. et al. MYC2 orchestrates a hierarchical transcriptional cascade that regulates jasmonate-mediated plant immunity in tomato Plant Cell, 29 (2017),pp. 1883-1906
[6]	Feng, Z.Y., Zhang, B.T., Ding, W.N. et al. Efficient genome editing in plants using a CRISPR/Cas system Cell Res., 23 (2013),pp. 1229-1232
[7]	Fernandez-Moreno, J.P., Tzfadia, O., Forment, J. et al. Characterization of a new pink-fruited tomato mutant results in the identification of a null allele of the SlMYB12 transcription factor Plant Physiol., 171 (2016),pp. 1821-1836
[8]	Gao, X., Chen, J., Dai, X. et al. Plant Physiol., 171 (2016),pp. 1794-1800
[9]	He, Y., Zhang, T., Yang, N. et al. Self-cleaving ribozymes enable the production of guide RNAs from unlimited choices of promoters for CRISPR/Cas9 mediated genome editing J. Genet. Genomics, 44 (2017),pp. 469-472
[10]	Jiang, W., Zhou, H., Bi, H. et al. Nucleic Acids Res., 41 (2013),p. e188
[11]	Li, J., Sun, Y., Du, J. et al. Generation of targeted point mutations in rice by a modified CRISPR/Cas9 system Mol. Plant, 10 (2017),pp. 526-529
[12]	Li, J., Zhang, H., Si, X. et al. J. Genet. Genomics, 44 (2017),pp. 465-468
[13]	Lin, T., Zhu, G., Zhang, J. et al. Genomic analyses provide insights into the history of tomato breeding Nat. Genet., 46 (2014),pp. 1220-1226
[14]	Miao, J., Guo, D.S., Zhang, J.Z. et al. Targeted mutagenesis in rice using CRISPR-Cas system Cell Res., 23 (2013),pp. 1233-1236
[15]	Shan, Q.W., Wang, Y.P., Li, J. et al. Targeted genome modification of crop plants using a CRISPR-Cas system Nat. Biotechnol., 31 (2013),pp. 686-688
[16]	The Tomato Genome Consortium The tomato genome sequence provides insights into fleshy fruit evolution Nature, 485 (2012),pp. 635-641
[17]	Wang, Y., Cheng, X., Shan, Q. et al. Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew Nat. Biotechnol., 32 (2014),pp. 947-951
[18]	Wang, Z.P., Xing, H.L., Dong, L. et al. Genome Biol., 16 (2015),p. 144
[19]	Xie, K., Yang, Y. RNA-guided genome editing in plants using a CRISPR-Cas system Mol. Plant, 6 (2013),pp. 1975-1983
[20]	Zhang, H., Zhang, J., Wei, P. et al. The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation Plant Biotechnol. J., 12 (2014),pp. 797-807