Generation of ApoE deficient dogs via combination of embryo injection of CRISPR/Cas9 with somatic cell nuclear transfer

Chong Feng^{1, #},
Xiaomin Wang^{2, 3, #},
Hui Shi^{2, 3, #},
Quanmei Yan⁶,
Min Zheng⁷,
Jing Li⁷,
Quanjun Zhang⁹,
Yumin Qin¹⁰,
Yougang Zhong^{11
, ,},
Jidong Mi^{12
, ,},
Liangxue Lai^{13, 14
, ,}

1.
Beijing SINOGENE Biotechnology Co., Ltd, Beijing 102200, China
2.
Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
3.
University of Chinese Academy of Sciences, Beijing 100049, China
6.
Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
7.
Beijing SINOGENE Biotechnology Co., Ltd, Beijing 102200, China
9.
Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
10.
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
11.
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
12.
Beijing SINOGENE Biotechnology Co., Ltd, Beijing 102200, China
13.
Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
14.
Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Veterinary Medicine, Jilin University, Changchun 130062, China

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Corresponding author: E-mail address: 18911223116@126.com (Yougang Zhong); E-mail address: jidong-m@sinogene.com.cn (Jidong Mi); E-mail address: lai_liangxue@gibh.ac.cn (Liangxue Lai)

These authors contributed equally to this work.

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These authors contributed equally to this work.

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[1]	Gofman, J.W., Lindgren, F. The role of lipids and lipoproteins in atherosclerosis Science, 111 (1950),pp. 166-171
[2]	Hong, S.G., Jang, G., Kim, M.K. et al. Dogs cloned from fetal fibroblasts by nuclear transfer Anim. Reprod. Sci., 115 (2009),pp. 334-339
[3]	Langheinrich, A.C., Michniewicz, A., Bohle, R.M. et al. Atherosclerosis, 191 (2007),pp. 73-81
[4]	Lusis, A.J. Atherosclerosis Nature, 407 (2000),pp. 233-241
[5]	Niemann, H., Lucas-Hahn, A. Somatic cell nuclear transfer cloning: practical applications and current legislation Reprod. Domest. Anim., 47 (2012),pp. 2-10
[6]	Piedrahita, J.A., Zhang, S.H., Hagaman, J.R. et al. Generation of mice carrying a mutant apolipoprotein E gene inactivated by gene targeting in embryonic stem-cells Proc. Natl. Acad. Sci. U. S. A., 89 (1992),pp. 4471-4475
[7]	Plump, A.S., Smith, J.D., Hayek, T. et al. Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells Cell, 71 (1992),pp. 343-353
[8]	Tsai, K.L., Clark, L.A., Murphy, K.E. Understanding hereditary diseases using the dog and human as companion model systems Mamm. Genome, 18 (2007),pp. 444-451
[9]	Zhang, S.H., Reddick, R.L., Piedrahita, J.A. et al. Spontaneous hypercholesterolemia and arterial lesions in mice lacking apolipoprotein E Science, 258 (1992),pp. 468-471
[10]	Zou, Q.J., Wang, X.M., Liu, Y.Z. et al. Generation of gene-target dogs using CRISPR/Cas9 system J. Mol. Cell Biol., 7 (2015),pp. 580-583

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doi: 10.1016/j.jgg.2017.11.003

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Generation of ApoE deficient dogs via combination of embryo injection of CRISPR/Cas9 with somatic cell nuclear transfer

Corresponding author: E-mail address: 18911223116@126.com (Yougang Zhong); E-mail address: jidong-m@sinogene.com.cn (Jidong Mi); E-mail address: lai_liangxue@gibh.ac.cn (Liangxue Lai)

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doi: 10.1016/j.jgg.2017.11.003

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Generation of ApoE deficient dogs via combination of embryo injection of CRISPR/Cas9 with somatic cell nuclear transfer

Corresponding author: E-mail address: 18911223116@126.com (Yougang Zhong); E-mail address: jidong-m@sinogene.com.cn (Jidong Mi); E-mail address: lai_liangxue@gibh.ac.cn (Liangxue Lai)

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