Arabidopsis ENOR3 regulates RNAi-mediated antiviral defense

a.
Tsinghua-Peking Joint Center for Life Sciences, and MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing 100084, China
b.
Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521, USA
c.
Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Sciences, Capital Normal University, Beijing 100048, China

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Corresponding author: E-mail address: qitiancong@163.com (Tiancong Qi); E-mail address: shou-wei.ding@ucr.edu (Shou-Wei Ding); E-mail address: daoxinlab@tsinghua.edu.cn (Daoxin Xie)

These authors contributed equally to this work.

摘要

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Abstract: Viruses can infect host plants to cause severe diseases and substantial agricultural loss, while plants have evolved RNA interference (RNAi) strategy to defend against viral infection. Despite enormous efforts, only a few host proteins in RNAi pathway were shown to mediate antiviral defense, including RNA-dependent RNA polymerase 1 (RDR1), RDR6, DICER-LIKE 2 (DCL2) and DCL4. In this study, we carried out a genetic screen for antiviral factors of RNAi pathway in Arabidopsis rdr6 background via inoculation with a 2b-deficient Cucumber Mosaic Virus (CMV-Δ2b). We identified a mutant susceptible to CMV-Δ2b, referred to as (enor) 3-1 rdr6, and found that ENOR3 encodes a functionally unknown protein with high homology to the mammalian Non Imprinted in Prader-Willi/Angelman (NIPA) magnesium transporters. ENOR3 inhibits accumulation of CMV-Δ2b and acts additively with RDR1, RDR6, DCL2 and DCL4 in antiviral defense. These results uncover that ENOR3 is a key component in antiviral RNAi pathway, and provide new insights into antiviral immunity.
- Antiviral defense /
- CMV-Δ2b /
- DCL2/4 /
- ENOR3 /
- RDR1/6 /
- RNAi
These authors contributed equally to this work.
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