Optimized Target-AID system efficiently induces single base changes in zebrafish

Xiaochan Lu^a,
Yunxing Liu^a,
Guanrong Yan^a,
Song Li^a,
Wei Qin^{a
, ,},
Shuo Lin^{a, b
, ,}

a.
State Key Laboratory of Chemical Oncogenomics, Key Laboratory of Chemical Genomics, Peking University Shenzhen Graduate School, Shenzhen 518055, China
b.
Department of Molecular, Cell and Development Biology, University of California, Los Angeles, Los Angeles, CA 90095, USA

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Corresponding author: E-mail address: qinwei@pkusz.edu.cn (Wei Qin); E-mail address: shuolin@ucla.edu (Shuo Lin)

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[1]	Cong, L., Ran, F.A., Cox, D. et al. Multiplex genome engineering using CRISPR/VCas systems Science, 339 (2013),pp. 819-823
[2]	Hwang, W.Y., Fu, Y., Reyon, D. et al. Nat. Biotechnol., 31 (2013),pp. 227-229
[3]	Jao, L.-E., Wente, S.R., Chen, W. Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system Proc. Natl. Acad. Sci. U. S. A., 110 (2013),pp. 13904-13909
[4]	Kim, K., Ryu, S.-M., Kim, S.-T. et al. Highly efficient RNA-guided base editing in mouse embryos Nat. Biotechnol., 35 (2017),pp. 435-437
[5]	Komor, A.C., Kim, Y.B., Packer, M.S. et al. Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage Nature, 533 (2016),pp. 420-424
[6]	Komor, A.C., Zhao, K.T., Packer, M.S. et al. Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C: G-to-T: A base editors with higher efficiency and product purity Sci. Adv., 3 (2017)
[7]	Liang, P., Sun, H., Sun, Y. et al. Effective gene editing by high-fidelity base editor 2 in mouse zygotes Protein Cell, 8 (2017),pp. 601-611
[8]	Nishida, K., Arazoe, T., Yachie, N. et al. Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems Science, 353 (2016)
[9]	Sander, J.D., Joung, J.K. CRISPR-Cas systems for editing, regulating and targeting genomes Nat. Biotechnol., 32 (2014),pp. 347-355
[10]	Shimatani, Z., Kashojiya, S., Takayama, M. et al. Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion Nat. Biotechnol., 35 (2017),pp. 441-443
[11]	Zhang, Y., Qin, W., Lu, X. et al. Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system Nat. Commun., 8 (2017),p. 118
[12]	Zong, Y., Wang, Y., Li, C. et al. Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion Nat. Biotechnol., 35 (2017),pp. 438-440
[13]	Zu, Y., Tong, X., Wang, Z. et al. TALEN-mediated precise genome modification by homologous recombination in zebrafish Nat. Methods, 10 (2013),pp. 329-331

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doi: 10.1016/j.jgg.2018.01.008

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Optimized Target-AID system efficiently induces single base changes in zebrafish

Corresponding author: E-mail address: qinwei@pkusz.edu.cn (Wei Qin); E-mail address: shuolin@ucla.edu (Shuo Lin)

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doi: 10.1016/j.jgg.2018.01.008

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Optimized Target-AID system efficiently induces single base changes in zebrafish

Corresponding author: E-mail address: qinwei@pkusz.edu.cn (Wei Qin); E-mail address: shuolin@ucla.edu (Shuo Lin)

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